[galaxy-commits] galaxy-dist commit eee0e1d344d2: Update Tophat and Cufflinks tool wrappers and functional tests to support Tophat version 1.1.* This breaks support for Tophat versions 1.0.* and earlier.

20 Nov 2010

# HG changeset patch -- Bitbucket.org
# Project galaxy-dist
# URL http://bitbucket.org/galaxy/galaxy-dist/overview
# User jeremy goecks <jeremy.goecks@emory.edu>
# Date 1289247694 18000
# Node ID eee0e1d344d21de7fb079fcccc3df8e251d1e025
# Parent  1ebd342fb4eb29c095b19a960503402f75cbe944
Update Tophat and Cufflinks tool wrappers and functional tests to support Tophat version 1.1.*  This breaks support for Tophat versions 1.0.* and earlier.

--- a/test-data/tophat_out2.wig
+++ /dev/null
@@ -1,175 +0,0 @@
-track type=bedGraph name="TopHat - read coverage"
-test_chromosome	0	52	0
-test_chromosome	52	53	1
-test_chromosome	53	54	2
-test_chromosome	54	55	3
-test_chromosome	55	57	4
-test_chromosome	57	58	6
-test_chromosome	58	60	7
-test_chromosome	60	65	8
-test_chromosome	65	70	9
-test_chromosome	70	71	10
-test_chromosome	71	72	11
-test_chromosome	72	75	12
-test_chromosome	75	77	13
-test_chromosome	77	79	15
-test_chromosome	79	82	16
-test_chromosome	82	85	17
-test_chromosome	85	87	19
-test_chromosome	87	88	20
-test_chromosome	88	90	21
-test_chromosome	90	91	22
-test_chromosome	91	93	23
-test_chromosome	93	95	26
-test_chromosome	95	97	28
-test_chromosome	97	99	30
-test_chromosome	99	100	31
-test_chromosome	100	101	35
-test_chromosome	101	102	36
-test_chromosome	102	105	38
-test_chromosome	105	106	39
-test_chromosome	106	107	40
-test_chromosome	107	112	43
-test_chromosome	112	114	44
-test_chromosome	114	118	45
-test_chromosome	118	121	46
-test_chromosome	121	122	48
-test_chromosome	122	124	50
-test_chromosome	124	126	53
-test_chromosome	126	129	54
-test_chromosome	129	130	53
-test_chromosome	130	131	55
-test_chromosome	131	132	57
-test_chromosome	132	133	55
-test_chromosome	133	134	54
-test_chromosome	134	135	55
-test_chromosome	135	136	54
-test_chromosome	136	137	56
-test_chromosome	137	138	57
-test_chromosome	138	141	60
-test_chromosome	141	147	62
-test_chromosome	147	148	61
-test_chromosome	148	150	62
-test_chromosome	150	152	61
-test_chromosome	152	153	60
-test_chromosome	153	154	61
-test_chromosome	154	155	63
-test_chromosome	155	156	64
-test_chromosome	156	158	65
-test_chromosome	158	159	66
-test_chromosome	159	160	68
-test_chromosome	160	161	67
-test_chromosome	161	163	68
-test_chromosome	163	164	69
-test_chromosome	164	165	71
-test_chromosome	165	167	73
-test_chromosome	167	168	74
-test_chromosome	168	169	72
-test_chromosome	169	170	75
-test_chromosome	170	171	73
-test_chromosome	171	172	74
-test_chromosome	172	174	75
-test_chromosome	174	175	77
-test_chromosome	175	176	74
-test_chromosome	176	177	73
-test_chromosome	177	181	71
-test_chromosome	181	182	70
-test_chromosome	182	183	67
-test_chromosome	183	184	68
-test_chromosome	184	189	69
-test_chromosome	189	193	68
-test_chromosome	193	194	69
-test_chromosome	194	195	71
-test_chromosome	195	196	72
-test_chromosome	196	197	70
-test_chromosome	197	199	68
-test_chromosome	199	200	66
-test_chromosome	200	201	67
-test_chromosome	201	202	66
-test_chromosome	202	204	65
-test_chromosome	204	205	67
-test_chromosome	205	207	65
-test_chromosome	207	211	66
-test_chromosome	211	213	65
-test_chromosome	213	216	64
-test_chromosome	216	220	62
-test_chromosome	220	224	63
-test_chromosome	224	225	64
-test_chromosome	225	228	66
-test_chromosome	228	229	65
-test_chromosome	229	230	62
-test_chromosome	230	231	61
-test_chromosome	231	232	60
-test_chromosome	232	234	59
-test_chromosome	234	237	58
-test_chromosome	237	238	57
-test_chromosome	238	239	55
-test_chromosome	239	240	53
-test_chromosome	240	241	50
-test_chromosome	241	242	51
-test_chromosome	242	243	52
-test_chromosome	243	244	53
-test_chromosome	244	246	50
-test_chromosome	246	247	52
-test_chromosome	247	249	50
-test_chromosome	249	250	47
-test_chromosome	250	349	0
-test_chromosome	349	350	1
-test_chromosome	350	351	49
-test_chromosome	351	352	51
-test_chromosome	352	353	53
-test_chromosome	353	354	54
-test_chromosome	354	357	55
-test_chromosome	357	358	56
-test_chromosome	358	361	55
-test_chromosome	361	362	57
-test_chromosome	362	365	56
-test_chromosome	365	366	57
-test_chromosome	366	368	58
-test_chromosome	368	369	57
-test_chromosome	369	372	56
-test_chromosome	372	375	59
-test_chromosome	375	378	60
-test_chromosome	378	379	59
-test_chromosome	379	380	57
-test_chromosome	380	381	56
-test_chromosome	381	382	54
-test_chromosome	382	384	53
-test_chromosome	384	386	52
-test_chromosome	386	387	51
-test_chromosome	387	388	50
-test_chromosome	388	390	48
-test_chromosome	390	395	47
-test_chromosome	395	396	45
-test_chromosome	396	398	44
-test_chromosome	398	399	43
-test_chromosome	399	400	42
-test_chromosome	400	402	1
-test_chromosome	402	500	0
-test_chromosome	500	508	39
-test_chromosome	508	509	38
-test_chromosome	509	510	37
-test_chromosome	510	511	36
-test_chromosome	511	516	35
-test_chromosome	516	517	33
-test_chromosome	517	518	31
-test_chromosome	518	521	29
-test_chromosome	521	522	26
-test_chromosome	522	524	25
-test_chromosome	524	525	24
-test_chromosome	525	526	22
-test_chromosome	526	527	20
-test_chromosome	527	528	18
-test_chromosome	528	529	17
-test_chromosome	529	530	16
-test_chromosome	530	532	15
-test_chromosome	532	534	14
-test_chromosome	534	536	13
-test_chromosome	536	540	11
-test_chromosome	540	541	10
-test_chromosome	541	543	9
-test_chromosome	543	544	8
-test_chromosome	544	545	7
-test_chromosome	545	547	6
-test_chromosome	547	549	3
-test_chromosome	549	549	2

--- a/tools/ngs_rna/tophat_wrapper.py
+++ b/tools/ngs_rna/tophat_wrapper.py
@@ -10,9 +10,8 @@ def __main__():
     #Parse Command Line
     parser = optparse.OptionParser()
     parser.add_option( '-p', '--num-threads', dest='num_threads', help='Use this many threads to align reads. The default is 1.' )
-    parser.add_option( '-C', '--coverage-output', dest='coverage_output_file', help='Coverage output file; formate is WIG.' )
     parser.add_option( '-J', '--junctions-output', dest='junctions_output_file', help='Junctions output file; formate is BED.' )
-    parser.add_option( '-H', '--hits-output', dest='accepted_hits_output_file', help='Accepted hits output file; formate is SAM.' )
+    parser.add_option( '-H', '--hits-output', dest='accepted_hits_output_file', help='Accepted hits output file; formate is BAM.' )
     parser.add_option( '', '--own-file', dest='own_file', help='' )
     parser.add_option( '-D', '--indexes-path', dest='index_path', help='Indexes directory; location of .ebwt and .fa files.' )
     parser.add_option( '-r', '--mate-inner-dist', dest='mate_inner_dist', help='This is the expected (mean) inner distance between mate pairs. \
@@ -186,33 +185,14 @@ def __main__():
         tmp_stderr.close()
         if returncode != 0:
             raise Exception, stderr
+        
+        # TODO: look for errors in program output.
+        
+        # Copy output files from tmp directory to specified files. 
+        shutil.copyfile( os.path.join( tmp_output_dir, "junctions.bed" ), options.junctions_output_file )
+        shutil.copyfile( os.path.join( tmp_output_dir, "accepted_hits.bam" ), options.accepted_hits_output_file )
     except Exception, e:
-        # Clean up temp dirs
-        if os.path.exists( tmp_output_dir ):
-            shutil.rmtree( tmp_output_dir )
-        stop_err( 'Error in tophat:\n' + str( e ) )
-        
-    # TODO: look for errors in program output.
-        
-    # Postprocessing: copy output files from tmp directory to specified files. Also need to remove header lines from SAM file.
-    try:
-        try:
-            shutil.copyfile( os.path.join( tmp_output_dir, "coverage.wig" ), options.coverage_output_file )
-            shutil.copyfile( os.path.join( tmp_output_dir, "junctions.bed" ), options.junctions_output_file )
-            
-            # Remove headers from SAM file in place.
-            in_header = True # Headers always at start of file.
-            for line in fileinput.input( os.path.join( tmp_output_dir, "accepted_hits.sam" ), inplace=1 ):
-                if in_header and line.startswith("@"):
-                    continue
-                else:
-                    in_header = False
-                    sys.stdout.write( line )
-                    
-            # Copy SAM File.
-            shutil.copyfile( os.path.join( tmp_output_dir, "accepted_hits.sam" ), options.accepted_hits_output_file )
-        except Exception, e:
-            stop_err( 'Error in tophat:\n' + str( e ) ) 
+        stop_err( 'Error in tophat:\n' + str( e ) ) 
     finally:
         # Clean up temp dirs
         if os.path.exists( tmp_index_dir ):

--- a/test-data/tophat_out3.bed
+++ /dev/null
@@ -1,3 +0,0 @@
-track name=junctions description="TopHat junctions"
-test_chromosome	180	402	JUNC00000001	46	+	180	402	255,0,0	2	70,52	0,170
-test_chromosome	349	550	JUNC00000002	38	+	349	550	255,0,0	2	51,50	0,151

--- a/tools/ngs_rna/cuffdiff_wrapper.xml
+++ b/tools/ngs_rna/cuffdiff_wrapper.xml
@@ -33,8 +33,8 @@
     </command><inputs><param format="gtf" name="gtf_input" type="data" label="Transcripts" help="A transcript GTF file produced by cufflinks, cuffcompare, or other source."/>
-        <param format="sam" name="aligned_reads1" type="data" label="SAM file of aligned RNA-Seq reads" help=""/>
-        <param format="sam" name="aligned_reads2" type="data" label="SAM file of aligned RNA-Seq reads" help=""/>
+        <param format="sam,bam" name="aligned_reads1" type="data" label="SAM or BAM file of aligned RNA-Seq reads" help=""/>
+        <param format="sam,bam" name="aligned_reads2" type="data" label="SAM or BAM file of aligned RNA-Seq reads" help=""/><param name="fdr" type="float" value="0.05" label="False Discovery Rate" help="The allowed false discovery rate."/><param name="min_mapqual" type="integer" value="0" label="Min SAM Mapping Quality" help="Instructs Cufflinks to ignore alignments with a SAM mapping quality lower than this number."/><param name="min_alignment_count" type="integer" value="0" label="Min Alignment Count" help="The minimum number of alignments in a locus for needed to conduct significance testing on changes in that locus observed between samples."/>

--- /dev/null
+++ b/test-data/tophat_out1.bed
@@ -0,0 +1,3 @@
+track name=junctions description="TopHat junctions"
+test_chromosome	180	402	JUNC00000001	46	+	180	402	255,0,0	2	70,52	0,170
+test_chromosome	349	550	JUNC00000002	38	+	349	550	255,0,0	2	51,50	0,151

--- a/tools/ngs_rna/cufflinks_wrapper.xml
+++ b/tools/ngs_rna/cufflinks_wrapper.xml
@@ -23,7 +23,7 @@
             #end if
     </command><inputs>
-        <param format="sam" name="input" type="data" label="SAM file of aligned RNA-Seq reads" help=""/>
+        <param format="sam,bam" name="input" type="data" label="SAM or BAM file of aligned RNA-Seq reads" help=""/><param name="max_intron_len" type="integer" value="300000" label="Max Intron Length" help=""/><param name="min_isoform_fraction" type="float" value="0.05" label="Min Isoform Fraction" help=""/><param name="pre_mrna_fraction" type="float" value="0.05" label="Pre MRNA Fraction" help=""/>

--- a/tools/ngs_rna/tophat_wrapper.xml
+++ b/tools/ngs_rna/tophat_wrapper.xml
@@ -1,15 +1,14 @@
-<tool id="tophat" name="Tophat" version="1.1.1">
+<tool id="tophat" name="Tophat" version="1.1.2"><description>Find splice junctions using RNA-seq data</description><requirements><requirement type="package">tophat</requirement></requirements><command interpreter="python">
         tophat_wrapper.py
-            ## Change this to accomodate the number of threads you have available.
+            ## Change this to accommodate the number of threads you have available.
             --num-threads="4"
             
             ## Provide outputs.
-            --coverage-output=$coverage
             --junctions-output=$junctions
             --hits-output=$accepted_hits
             
@@ -338,9 +337,8 @@
     </inputs><outputs>
-        <data format="sam" name="accepted_hits" label="${tool.name} on ${on_string}: accepted_hits"/>
-        <data format="bedgraph" name="coverage" label="${tool.name} on ${on_string}: coverage"/><data format="bed" name="junctions" label="${tool.name} on ${on_string}: splice junctions"/>
+        <data format="bam" name="accepted_hits" label="${tool.name} on ${on_string}: accepted_hits"/></outputs><tests>
@@ -367,9 +365,9 @@
             <param name="input2" ftype="fastqsanger" value="tophat_in2.fq"/><param name="mate_inner_distance" value="20"/><param name="pSettingsType" value="preSet"/>
-            <output name="accepted_hits" file="tophat_out1.sam" sort="True"/>
-            <output name="coverage" file="tophat_out2.wig"/>
-            <output name="junctions" file="tophat_out3.bed"/>
+            <output name="junctions" file="tophat_out1.bed"/>
+            <!-- Bam files always differ (due to magic number?), so can't test this right now. -->
+            <output name="accepted_hits" file="tophat_out2.bam" lines_diff="100000"/></test><!--        <test><param name="genomeSource" value="history"/>
@@ -451,15 +449,13 @@ Tophat accepts files in Sanger FASTQ for
 
 **Outputs**
 
-Tophat produces three output files:
+Tophat produces two output files:
 
-- coverage.wig -- A UCSC BedGraph_ wigglegram track, showing the depth of coverage at each position, including the spliced read alignments.
-- accepted_hits.sam -- A list of read alignments in SAM_ format.
-- junctions.bed -- A UCSC BED_ track of junctions reported by TopHat. Each junction consists of two connected BED blocks, where each block is as long as the maximal overhang of any read spanning the junction. The score is the number of alignments spanning the junction.
-  
-.. _BedGraph: http://genome.ucsc.edu/goldenPath/help/bedgraph.html
-.. _SAM: http://samtools.sourceforge.net/
+- junctions -- A UCSC BED_ track of junctions reported by TopHat. Each junction consists of two connected BED blocks, where each block is as long as the maximal overhang of any read spanning the junction. The score is the number of alignments spanning the junction.
+- accepted_hits -- A list of read alignments in BAM_ format.
+
 .. _BED: http://genome.ucsc.edu/FAQ/FAQformat.html#format1
+.. _BAM: http://samtools.sourceforge.net/
     
 -------
 

--- a/test-data/tophat_out1.sam
+++ /dev/null
@@ -1,179 +0,0 @@
-test_mRNA_103_284_2a	161	test_chromosome	153	255	75M	=	360	0	CGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_103_284_2a	81	test_chromosome	360	255	41M100N34M	=	153	0	TTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACTTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
-test_mRNA_104_274_1c	73	test_chromosome	350	255	51M100N24M	*	0	0	CACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_104_278_3e	161	test_chromosome	154	255	75M	=	354	0	GACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTTTTTGGCGCGCGGCCCTACGGCTG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_104_278_3e	81	test_chromosome	354	255	47M100N28M	=	154	0	ATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGAATCGAGGCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_105_266_13	163	test_chromosome	155	255	75M	=	242	0	ACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_105_266_13	83	test_chromosome	242	255	9M100N50M100N16M	=	155	0	CGATCCGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_105_276_c	161	test_chromosome	155	255	75M	=	352	0	ACTGGACTATTTAGGACGATCGGACTGAGGAAGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_105_276_c	81	test_chromosome	352	255	49M100N26M	=	155	0	CTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGGCTTTTTCTACTTGAGACTGGGATCGAGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_106_253_45	137	test_chromosome	156	255	75M	*	0	0	CTGGACTATTTAGGTCGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_107_286_5	161	test_chromosome	157	255	75M	=	362	0	TGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGCATTTGGCGCGCGGCCCTACGGCTGAGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_107_286_5	81	test_chromosome	362	255	39M100N36M	=	157	0	ATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
-test_mRNA_110_267_22	163	test_chromosome	160	255	75M	=	243	0	ACTAGTTAGGGCGATCGGACTGAGGAGGGCAGTAGGACGCTACGTAGTTGGCGCGCGGCCCTACGACTGAGCGTC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:5	NH:i:1
-test_mRNA_110_267_22	83	test_chromosome	243	255	8M100N50M100N17M	=	160	0	GATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
-test_mRNA_110_271_28	147	test_chromosome	247	255	4M100N50M100N21M	=	160	0	CGCCACTATTACTTTATTATCTTACTCGGACGAAGACGGATCGGCAACGGGGCTTTTTCTACTTGAGACTGGGAT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_110_271_28	99	test_chromosome	160	255	75M	=	247	0	ACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_111_268_d	73	test_chromosome	244	255	7M100N50M100N18M	*	0	0	ATACGCCACTATTATTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_111_297_61	161	test_chromosome	161	255	75M	=	373	0	CTATTTAGGACGATCGGACTGGGGAGGGCAGTAGGACGCTACGGATTTGGCGCGCGGCCCTACGGCTGAGCGTCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_111_297_61	81	test_chromosome	373	255	28M100N47M	=	161	0	CGGACGTAGACGGATCCGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTTAGGACGGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_114_277_5b	161	test_chromosome	164	255	75M	=	353	0	TTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGCCTGAGCGTCGAGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_114_277_5b	81	test_chromosome	353	255	48M100N27M	=	164	0	TATTACTTTATTATCTTACTCGGAGGTAGACGGAACGGCAACGGGACTTTTTCTGCTTGAGACTGGGATCGAGGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	XS:A:+	NH:i:1
-test_mRNA_116_271_2b	163	test_chromosome	166	255	75M	=	247	0	TAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_116_271_2b	83	test_chromosome	247	255	4M100N50M100N21M	=	166	0	CGCCACTATTACTTTATTATCTTACTCGGACGTAGACAGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGAT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_116_295_63	161	test_chromosome	166	255	75M	=	371	0	TAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_116_295_63	81	test_chromosome	371	255	30M100N45M	=	166	0	CTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTTAGGACGGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
-test_mRNA_118_297_f	161	test_chromosome	168	255	75M	=	373	0	GGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_118_297_f	81	test_chromosome	373	255	28M100N47M	=	168	0	CGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTTAGGACGGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
-test_mRNA_11_190_1a	147	test_chromosome	166	255	75M	=	61	0	TAGGTCGATGGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGTGGCCCTACGGCTGAGCGTCGAGCTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	NH:i:1
-test_mRNA_11_190_1a	99	test_chromosome	61	255	75M	=	166	0	GACTAGACTGGAGGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_122_299_6	161	test_chromosome	172	255	75M	=	375	0	GATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_122_299_6	81	test_chromosome	375	255	26M100N49M	=	172	0	GACGTAGACGGAGCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTTAGGACGGGACTTG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_125_280_48	145	test_chromosome	356	255	45M100N30M	=	175	0	TACTTTATTATCTTACTCTGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGAGCGAGGCGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_125_280_48	97	test_chromosome	175	255	75M	=	356	0	CGGACTGAGGAGGGCAGTAGGACGCTATGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGAAACGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_125_293_60	161	test_chromosome	175	255	75M	=	369	0	CGGACTGAGGAGGGCAGTAGGACGCTATGTATTTGGCGCGCGGCCCTACGGCTGAGCTTCGAGGTTGCGATACGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	NH:i:1
-test_mRNA_125_293_60	81	test_chromosome	369	255	32M100N43M	=	175	0	TACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTTAGGACGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
-test_mRNA_126_282_18	161	test_chromosome	176	255	75M	=	358	0	GGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_126_282_18	81	test_chromosome	358	255	43M100N32M	=	176	0	CTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
-test_mRNA_128_252_36	137	test_chromosome	228	255	23M100N52M	*	0	0	GAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGGAACGGGACTTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	XS:A:+	NH:i:1
-test_mRNA_131_260_33	147	test_chromosome	236	255	15M100N50M100N10M	=	181	0	AGCTTGTGATACGCCACTATTACTTTATTATCTTACTCGGACGTAAACGGATCGGCCACGGGACTTTTTTTACTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	XS:A:+	NH:i:1
-test_mRNA_131_260_33	99	test_chromosome	181	255	70M100N5M	=	236	0	GAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTAT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_145_300_37	163	test_chromosome	195	255	56M100N19M	=	376	0	GACGCTACGTATTTGGCGCGGGGCCCTATGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTAGTATATT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:5	XS:A:+	NH:i:1
-test_mRNA_145_300_37	83	test_chromosome	376	255	25M100N50M	=	195	0	ACGTAGACGGATCGGAAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTTAGGACGGGACTTGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_150_290_0	73	test_chromosome	366	255	35M100N40M	*	0	0	TCTTACTCGGACGTAGACGGATCGCCAACGGGACTTTTTCTACTTGAGACTGAGACCGAGGCGGACTTTTTAGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_151_286_e	137	test_chromosome	362	255	39M100N36M	*	0	0	ATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTATCTACTTGAGACTGGGATCGAGGCGGACTTTTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_151_297_1d	137	test_chromosome	373	255	28M100N47M	*	0	0	CGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACATTTTAGGACGGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_16_194_10	163	test_chromosome	66	255	75M	=	170	0	GACTGGATGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTCGGACTACGGACGGACTTAAAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_16_194_10	83	test_chromosome	170	255	75M	=	66	0	ACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_172_294_4f	147	test_chromosome	370	255	31M100N44M	=	222	0	ACTCGGACGTAGACGGGTCGGCAGCGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGGACGTTTTAGGACGGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_172_294_4f	99	test_chromosome	222	255	29M100N46M	=	370	0	ACGGATGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTCCTCGGACGTAGACGGATCGCCAACGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_21_208_24	163	test_chromosome	71	255	75M	=	184	0	GAGGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_21_208_24	83	test_chromosome	184	255	67M100N8M	=	71	0	GAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGCCTGAGCGTCGAGCTTGCGATACGCCACTATTAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_22_173_62	147	test_chromosome	149	255	75M	=	72	0	GCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_22_173_62	99	test_chromosome	72	255	75M	=	149	0	AGGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_23_186_42	163	test_chromosome	73	255	75M	=	162	0	GGCGCTTGTGACTGAGCTAGGACGTGCCACTACGGGGATGAAGACTAGGACTACGGACGGACTTAGAGCGTCAGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_23_186_42	83	test_chromosome	162	255	75M	=	73	0	TATTTAGGACGATCGGACGGAGGAGGGCAGAAGGACGCTACGTATTTGGCGCGCGGCCCTACGACTGAGCGTCGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	NH:i:1
-test_mRNA_26_189_30	163	test_chromosome	76	255	75M	=	165	0	GCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_26_189_30	83	test_chromosome	165	255	75M	=	76	0	TTAGGACGATCGGACTGAGGAGGGCAGTAGGACGGTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_28_188_11	147	test_chromosome	164	255	75M	=	78	0	TTTAGGACGATCGGACTGAGGAAGGCAGTAGGACGCTTCGTATTTGGCGCGAGGCCCTACGGCTGAGCGTCGAGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	NH:i:1
-test_mRNA_28_188_11	99	test_chromosome	78	255	75M	=	164	0	TTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGAACGGACTTAGAGCGTCAGATGCAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_28_206_1f	73	test_chromosome	78	255	75M	*	0	0	TTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGACGCAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_30_231_3c	161	test_chromosome	80	255	75M	=	207	0	GCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_30_231_3c	81	test_chromosome	207	255	44M100N31M	=	80	0	TTGGCGCGCGGCCCTACGGCTAAGCGTCGAGCTTGCGATACGCCACTATTACTTTAATATCTTACTCGCACGTAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	XS:A:+	NH:i:1
-test_mRNA_33_189_4a	73	test_chromosome	165	255	75M	*	0	0	TTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACCTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGGGCT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_33_223_4e	73	test_chromosome	83	255	75M	*	0	0	ACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_36_146_27	163	test_chromosome	86	255	75M	=	122	0	GCGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACAGACGGACTTAGAGCGTCAGATGCAGCGACTGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_36_146_27	83	test_chromosome	122	255	75M	=	86	0	ACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGTGCAGTAGGT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_36_218_12	147	test_chromosome	194	255	57M100N18M	=	86	0	GGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_36_218_12	99	test_chromosome	86	255	75M	=	194	0	GAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGCCTTAGAGCGTCAGATGCAGCGACTGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_38_199_29	147	test_chromosome	175	255	75M	=	88	0	CGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_38_199_29	99	test_chromosome	88	255	75M	=	175	0	GCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_39_219_5c	147	test_chromosome	195	255	56M100N19M	=	89	0	GACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCCAGCTTGCGATACGCCACTATTACTTTATTATCTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_39_219_5c	99	test_chromosome	89	255	75M	=	195	0	CTAGGACGTCCCACTATGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGGCTGGACTA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_3_187_51	147	test_chromosome	163	255	75M	=	53	0	ATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_3_187_51	99	test_chromosome	53	255	75M	=	163	0	TACTATTTGACTAGACTGGAGGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTCGGACTACG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_41_236_55	145	test_chromosome	212	255	39M100N36M	=	91	0	GCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_41_236_55	97	test_chromosome	91	255	75M	=	212	0	AGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGAATATT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_42_209_25	147	test_chromosome	185	255	66M100N9M	=	92	0	AGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_42_209_25	99	test_chromosome	92	255	75M	=	185	0	GGACGTGCCACTACGTGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_44_193_3f	147	test_chromosome	169	255	75M	=	94	0	GACGATCGGACTGGGGAGAGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_44_193_3f	99	test_chromosome	94	255	75M	=	169	0	ACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGTCTATTTAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_44_197_35	147	test_chromosome	173	255	75M	=	94	0	ATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGATCGTCGAGCTTGCGATAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_44_197_35	99	test_chromosome	94	255	75M	=	173	0	ACGTGCAACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_44_225_1e	163	test_chromosome	94	255	75M	=	201	0	ACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCGGGTGCAGCGACTGGACTATTTAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_44_225_1e	83	test_chromosome	201	255	50M100N25M	=	94	0	ACGTATATGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_46_195_17	137	test_chromosome	96	255	75M	*	0	0	GTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_46_232_2f	147	test_chromosome	208	255	43M100N32M	=	96	0	TGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_46_232_2f	99	test_chromosome	96	255	75M	=	208	0	GTGCCACTACGGGGATGACGACTAGGACTACGGCCGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_48_207_39	73	test_chromosome	98	255	75M	*	0	0	GCCCCTACGGGGATGACGACTAGGACTACGGACGGATTTAGACCGTCAGATGCAGCGACTGGACTATTTAGGACG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_48_249_20	161	test_chromosome	98	255	75M	=	225	0	GCCACTACGGGGATGACGACTAGGACGACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_48_249_20	81	test_chromosome	225	255	26M100N49M	=	98	0	GCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTACTATCTTACTCGGACGGAGACGGATCGGCAACGGGAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_4_191_5d	163	test_chromosome	54	255	75M	=	167	0	ACTATCTGACGAGACTGGAGGCGCTTGCGACTGAGCTAGGACGTACCATTACGCGGATGACGACTAGGACTACGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	NH:i:1
-test_mRNA_4_191_5d	83	test_chromosome	167	255	75M	=	54	0	AGGACGATCGGACTGAGTAGGGCAGTAGGACACTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_50_224_2d	163	test_chromosome	100	255	75M	=	200	0	CACTACGAGGATGACGTCTAGGACTACGGACGGACTTAGAGCGTCAGACGCAGCGACTGGACTATTTAGGACGAT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_50_224_2d	83	test_chromosome	200	255	51M100N24M	=	100	0	TACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_51_194_47	163	test_chromosome	101	255	75M	=	170	0	ACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_51_194_47	83	test_chromosome	170	255	75M	=	101	0	ACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_51_194_49	147	test_chromosome	170	255	75M	=	101	0	ACGTTCGGACTGAGGAGGGCAGTAGGACGCCACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	NH:i:1
-test_mRNA_51_194_49	99	test_chromosome	101	255	75M	=	170	0	ACTACGGGGATGACGACTAGGCCTACGGATGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_51_237_a	147	test_chromosome	213	255	38M100N37M	=	101	0	CGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGAT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_51_237_a	99	test_chromosome	101	255	75M	=	213	0	ACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_51_248_14	145	test_chromosome	224	255	27M100N48M	=	101	0	GGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGAACGGCAACGGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_51_248_14	97	test_chromosome	101	255	75M	=	224	0	ACTACGGGGATGACGACGAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGAACTTTTTAGGACGATC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_52_261_1b	145	test_chromosome	237	255	14M100N50M100N11M	=	102	0	GCTTGCGATACGCCACTATTACTTAATTATCTTACTCGGACGTAGAAGGATCGGCAACGGGACTTTTTCTACTTG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_52_261_1b	97	test_chromosome	102	255	75M	=	237	0	CTACGGGAATGACGACTAGGGCTACGGAGGGACTTACAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	NH:i:1
-test_mRNA_53_212_19	147	test_chromosome	188	255	63M100N12M	=	103	0	GCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTTCTTTA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_53_212_19	99	test_chromosome	103	255	75M	=	188	0	TACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGAATATTTAGGACGATCGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_53_272_5a	161	test_chromosome	103	255	75M	=	248	0	TACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_53_272_5a	81	test_chromosome	248	255	3M100N50M100N22M	=	103	0	GCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGACACTGGGATC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_56_183_56	147	test_chromosome	159	255	75M	=	106	0	GACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_56_183_56	99	test_chromosome	106	255	75M	=	159	0	GGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTGGGACGATCGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_57_231_8	147	test_chromosome	207	255	44M100N31M	=	107	0	TTGGCGCGCGGCCCTAGGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_57_231_8	99	test_chromosome	107	255	75M	=	207	0	GGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCACCGACTGGACTATTTAGGACGATCGGACTG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_58_218_16	163	test_chromosome	108	255	75M	=	194	0	GGATGACGACTAGGACTACGGACGGACTTAGAACGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_58_218_16	83	test_chromosome	194	255	57M100N18M	=	108	0	GGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_58_220_3d	163	test_chromosome	108	255	75M	=	196	0	GGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_58_220_3d	83	test_chromosome	196	255	55M100N20M	=	108	0	ACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGGTTGCGATACGCCACTATTACTTTATTATCTTC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_58_234_7	163	test_chromosome	108	255	75M	=	210	0	GGATGACGCCTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_58_234_7	83	test_chromosome	210	255	41M100N34M	=	108	0	GCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTAGTTTATTATCTGACTCGGACGTAGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	XS:A:+	NH:i:1
-test_mRNA_5_197_46	145	test_chromosome	173	255	75M	=	55	0	ATCGGACGGAGGAGGGCAGTAGGACGCTACGTATTTGGCGGGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_5_197_46	97	test_chromosome	55	255	75M	=	173	0	CTATCTGACTAGACTCGAGGCGCTTGCGTCTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_63_229_4c	163	test_chromosome	113	255	75M	=	205	0	ACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGGGACTGGACTATTTAGGACGATCGGACTGAGGAGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_63_229_4c	83	test_chromosome	205	255	46M100N29M	=	113	0	ATTTGGCGCGCGGCCCTACGGCTGAGTGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_65_238_2e	147	test_chromosome	214	255	37M100N38M	=	115	0	GCGGCCCTACGGCTGCGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_65_238_2e	99	test_chromosome	115	255	75M	=	214	0	GACTAGGACTACGGACGGACTTAGAGCGTCAGAAGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_69_229_23	163	test_chromosome	119	255	75M	=	205	0	AGGACTACGGACGGACTTATAGGGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_69_229_23	83	test_chromosome	205	255	46M100N29M	=	119	0	CTTTGGCGCGCGGCCCTACGGCTGAGCGTCTAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_6_182_59	147	test_chromosome	158	255	75M	=	56	0	GGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_6_182_59	99	test_chromosome	56	255	75M	=	158	0	TATCTGACTAGACTGGAGGCGCTTGCGACTGAGCTAGGACGTGCCAGTACGGGGATGACGACTAGGACTACGGAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_72_258_4	163	test_chromosome	122	255	75M	=	234	0	ACTACGGACGGACTTAGAGCGTCAGATGCAGCAACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_72_258_4	83	test_chromosome	234	255	17M100N50M100N8M	=	122	0	CGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATGGGCAACGGGACTTTTTCTAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_73_240_34	147	test_chromosome	216	255	35M100N40M	=	123	0	GGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTTCTCGGACGTAGACGGATCGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_73_240_34	99	test_chromosome	123	255	75M	=	216	0	CTACGGACGGACTTAGAGCGTCAGATGCAGCGAATGGACTATTTAGGACGCTCGGACTGAGGAGGGCAGTAGGAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_73_259_5e	147	test_chromosome	235	255	16M100N50M100N9M	=	123	0	GAGCTTGCGATACGCCACTATTACTGTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_73_259_5e	99	test_chromosome	123	255	75M	=	235	0	CTACGGACGGACTTAGAGCGTCAGATGCTGCGACTGGACTATTTGGGACGATCGGACTGAGGAGGGCAGTAGGAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_75_204_54	73	test_chromosome	125	255	75M	*	0	0	ACGGACGGACTTCGAGCCTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_75_235_21	73	test_chromosome	125	255	75M	*	0	0	ACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGCACGATCGGACTGAGGAGGGCAGTAGAACGT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_75_277_3b	145	test_chromosome	353	255	48M100N27M	=	125	0	TATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACCTGAGACTGGGATCGAGGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_75_277_3b	97	test_chromosome	125	255	75M	=	353	0	ACGGACGGACTTAAAGCTTCAGATGCAGCGACAGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_77_256_2c	73	test_chromosome	127	255	75M	*	0	0	GGACGGACTTAGAGCATCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_78_276_4b	145	test_chromosome	352	255	49M100N26M	=	128	0	CTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTAGGATCGAGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_78_276_4b	97	test_chromosome	128	255	75M	=	352	0	GACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGGCGCTAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_79_256_31	137	test_chromosome	129	255	75M	*	0	0	ACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_81_228_3a	163	test_chromosome	131	255	75M	=	204	0	GGACTGAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGTAGTAGGACGCTACGTA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_81_228_3a	83	test_chromosome	204	255	47M100N28M	=	131	0	TATTTGGCGCGCGGCCCTATGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGTAGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	XS:A:+	NH:i:1
-test_mRNA_81_245_4d	163	test_chromosome	131	255	75M	=	221	0	GGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGATGAGGGCAGTAGGACGCTACGTA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_81_245_4d	83	test_chromosome	221	255	30M100N45M	=	131	0	TACGGCTGAGCGTCGAGGTTGCGATACGCCACTATTACTTTATAATCTTACTCGGACGTAGACGGATCGGCAACG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_82_255_2	137	test_chromosome	132	255	75M	*	0	0	GACTTAGAGCGTCAGATGCAGCGACTGGACTTTTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTAT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_82_271_58	147	test_chromosome	247	255	4M100N50M100N21M	=	132	0	CGCCACTATTACTTTATTATCTTACTCGGACGTAGACGCATCGGCAACGGGACTTTTTCTACTTGAGACTGGGAT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_82_271_58	99	test_chromosome	132	255	75M	=	247	0	GACTTAGAGCGTCAGTTGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTAT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_85_268_53	147	test_chromosome	244	255	7M100N50M100N18M	=	135	0	ATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGTCAACGGGACTTTTTCTACTTGAGACTGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_85_268_53	99	test_chromosome	135	255	75M	=	244	0	TTAGTGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_85_275_38	137	test_chromosome	351	255	50M100N25M	*	0	0	ACTCTTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTACTACTTGAGACTGGGATCGAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_87_250_57	163	test_chromosome	137	255	75M	=	226	0	AGAGCGTCAGATGCAGAGACTGGACTATTTAGGACGATCGGACTGAGGAGTGCAGTAGGACGCTACGTATTTGGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_87_250_57	83	test_chromosome	226	255	25M100N50M	=	137	0	ATGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1
-test_mRNA_87_279_5f	161	test_chromosome	137	255	75M	=	355	0	AGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACCGAGGAGGGCAGTAGGACGCTACGTATTTGGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_87_279_5f	81	test_chromosome	355	255	46M100N29M	=	137	0	TTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGAGGCGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
-test_mRNA_88_257_50	137	test_chromosome	138	255	75M	*	0	0	GAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_89_230_b	163	test_chromosome	139	255	75M	=	206	0	AGCGTCAGGTGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_89_230_b	83	test_chromosome	206	255	45M100N30M	=	139	0	TCTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTAACTCACTCGGACGTA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	XS:A:+	NH:i:1
-test_mRNA_89_245_15	147	test_chromosome	221	255	30M100N45M	=	139	0	TACGGCTGAGCGTCGAGCTTGCGATACGCCACTATTTCTCTATTATCTTACTCGGACGTAGACGGATCGGCAACG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_89_245_15	99	test_chromosome	139	255	75M	=	221	0	AGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_89_267_32	163	test_chromosome	139	255	75M	=	243	0	AGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGAGTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_89_267_32	83	test_chromosome	243	255	8M100N50M100N17M	=	139	0	GATACGGCACTATTACTTTATTATCTTTCTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_8_155_9	163	test_chromosome	58	255	75M	=	131	0	TGTGACTAGACTGGAGGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_8_155_9	83	test_chromosome	131	255	75M	=	58	0	GGACTTCGAGCGTCAGATGCAGCGACTGTACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_8_197_1	147	test_chromosome	173	255	75M	=	58	0	ATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_8_197_1	99	test_chromosome	58	255	75M	=	173	0	TCTGACTAGACTGGAGGCGCTTGCGACTGAGCTAGGACGTGACACTACGGGGATGGCGACTAGGACTACGGACGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1
-test_mRNA_91_256_41	73	test_chromosome	141	255	75M	*	0	0	CGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_92_250_44	147	test_chromosome	226	255	25M100N50M	=	142	0	CTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGGTACGGGACT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
-test_mRNA_92_250_44	99	test_chromosome	142	255	75M	=	226	0	GTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
-test_mRNA_92_266_43	147	test_chromosome	242	255	9M100N50M100N16M	=	142	0	CGATACGCCACTATTACTTTCTTATCTTACTCGGACGTAGACGGAGCGGCAACGGGACTTTTTCTACTTGAGACC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_92_266_43	99	test_chromosome	142	255	75M	=	242	0	GTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTCAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_94_291_40	137	test_chromosome	367	255	34M100N41M	*	0	0	CTTCCTGGGACGTAGACGGATCGGCAACGCGACATTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTTGGGAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:5	XS:A:+	NH:i:1
-test_mRNA_96_238_3	163	test_chromosome	146	255	75M	=	214	0	GATGCAGCGACTGGACTATTTAGGACGATCGGACGGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGACC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
-test_mRNA_96_238_3	83	test_chromosome	214	255	37M100N38M	=	146	0	GCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCTACTAGTACTTTATTATCTTACGCGGACGTAGACGGATC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:4	XS:A:+	NH:i:1
-test_mRNA_97_275_26	145	test_chromosome	351	255	50M100N25M	=	147	0	ACTATTACTTTATTATCTTAGTCGGACGTAGACGGATCGGAAACGGGACTCTTTCTACTTGAGACTGGGATCGAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	XS:A:+	NH:i:1
-test_mRNA_97_275_26	97	test_chromosome	147	255	75M	=	351	0	ATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_9_179_52	163	test_chromosome	59	255	75M	=	155	0	CTGACTAGACTGGAGGCGCTCGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	NH:i:1
-test_mRNA_9_179_52	83	test_chromosome	155	255	75M	=	59	0	ACTGGACCATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	NH:i:1

Binary file test-data/tophat_out2.bam has changed

    

[galaxy-commits] galaxy-dist commit eee0e1d344d2: Update Tophat and Cufflinks tool wrappers and functional tests to support Tophat version 1.1.* This breaks support for Tophat versions 1.0.* and earlier.

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