Hi We have set up galaxy in a production environment and our galaxy users can connect to the FTP and upload files. No worries. The settings in the universe file seem correct and are. ftp_upload_dir = /home/galaxy/galaxy-dist/database/ftp It creates the directory and uploads the file. But when the user signs into galaxy they cannot view their file in the get data -> from files on my computer. Proftp config is listed below. Cheers James Boocock ServerName "Public Galaxy FTP" ServerType standalone DefaultServer on Port 21 Umask 077 SyslogFacility DAEMON SyslogLevel debug MaxInstances 30 User nobody Group nogroup DisplayConnect /etc/opt/local/proftpd_welcome.txt # Passive port range for the firewall PassivePorts 30000 40000 # Cause every FTP user to be "jailed" (chrooted) into their home directory DefaultRoot ~ # Automatically create home directory if it doesn't exist CreateHome on dirmode 1700 # Allow users to overwrite their files AllowOverwrite on # Allow users to resume interrupted uploads AllowStoreRestart on # Bar use of SITE CHMOD <Limit SITE_CHMOD> DenyAll </Limit> # Bar use of RETR (download) since this is not a public file drop <Limit RETR> DenyAll # Do not authenticate against real (system) users AuthPAM off # Set up mod_sql_password - Galaxy passwords are stored as hex-encoded SHA1 SQLPasswordEngine on SQLPasswordEncoding hex # Set up mod_sql to authenticate against the Galaxy database SQLEngine on SQLBackend postgres SQLConnectInfo galaxydb galaxyftp 1234 SQLAuthTypes SHA1 SQLAuthenticate users # An empty directory in case chroot fails SQLDefaultHomedir /var/opt/local/proftpd # Define a custom query for lookup that returns a passwd-like entry. UID and GID should match your Galaxy user. # Do not authenticate against real (system) users AuthPAM off # Set up mod_sql_password - Galaxy passwords are stored as hex-encoded SHA1 SQLPasswordEngine on SQLPasswordEncoding hex # Set up mod_sql to authenticate against the Galaxy database SQLEngine on SQLBackend postgres SQLConnectInfo galaxydb galaxyftp 1234 SQLAuthTypes SHA1 SQLAuthenticate users # An empty directory in case chroot fails SQLDefaultHomedir /var/opt/local/proftpd # Define a custom query for lookup that returns a passwd-like entry. UID and GID should match your Galaxy user. SQLUserInfo custom:/LookupGalaxyUser SQLNamedQuery LookupGalaxyUser SELECT "email,password,'512','512','/home/galaxy/galaxy-dist/database/ftp/%U','/bin/bash' FROM galaxy_user WHERE email='%U'" SQLUserInfo custom:/LookupGalaxyUser SQLNamedQuery LookupGalaxyUser SELECT "email,password,'512','512','/home/galaxy/galaxy-dist/database/ftp/%U','/bin/bash' FROM galaxy_user WHERE email='%U'" </Limit>