Hello, You probably need to reassign the datatype to be "fastqsanger", but double check the quality score scaling first. You FastQC run will be part of this. Our wiki has the details here, sections 2.9 & 2.10.1 http://wiki.galaxyproject.org/Support Best, Jen Galaxy team On Wed, Feb 11, 2015 at 10:10 AM, Xiong, Min, <mxiong2@cmh.edu> wrote:
Hi,
When I use BWA alignment, FASTQ file can't be detected. What reason cause it?
The attached file contains figure from galaxy, you will see I have good fastq file which can be reported by FastQC.
Thanks
Min Xiong, Ph.D.
Post-doctoral Fellow
Shui Q. Ye Lab Group
Division of Experimental and Translational Genetics
Department of Pediatrics
Children's Mercy Hospital
2401 Gillham Road
Kansas City, MO 64108
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