I have been struggling with setting up my local Galaxy instance for the past two days. Can anyone post a copy of their universe_wsgi.ini file , with the sensitive info commented / removed? I need an instance that can handle local files greater than 2 GB (ie: does not go through POST method of browser to get data) and has permissions to use pre-built bowtie indices. Also, a FUNCTIONNAL copy of the 'bowtie_indices.loc' would be very helpful. (none of my prebuilt databases are appearing in drop down menu). Please assist. Daniel Brami Synthetic Genomics, Inc. Senior Research Associate, Bioinformatics 11149 North Torrey Pines Road La Jolla, California 92037 Phone: 858.433.2230 Fax: 858.754.2988 DBrami@SyntheticGenomics.com<mailto:DBrami@SyntheticGenomics.com> www.SyntheticGenomics.com<http://www.syntheticgenomics.com/>