Hi Peter,

> What is bad is converting FASTA without qualities to FASTQ with made up
> qualities, regardless of if this is sequence or color space.
I am a newbie to this. I didn't know about this.

I was under the impression that 'conversion is bad whether one has the qual files or not!'

Thanks for the input.

> Do you have the quality scores for your data, in a *.qual file normally.

Yes, I do have the *.qual files with me.

I've tried to convert my data into FASTQ on my local installation but I was unsuccessful.

I've used this tool under NGS:QC and manipulation,

and used my *.qual & *.csfasta files as an input.

The tool didn't give me any error, but there was nothing inside the data set that is generated.

PFA for further info from the Inline image 4

button.

So, could you pls suggest me something on what to do about this ?

Thanks,

Nikhil.