Hi Carlos, It worked perfectly last time. But now on the new Ubuntu 14.04, no file called default exists under the folder sites-available, instead there are two files: 000-default.conf and default-ssl.conf. From the content of the files, 000-default.conf is more likely to be the alternative. I inserted directives as before and restart the apache service. The web browser told me that “Permission denied” even the galaxy-dist folder and its subfolder have at least 744 permission (everyone should be able to read). How can I solve this “Permission denied” issue? Best regards! Jun From: Carlos Borroto [mailto:carlos.borroto@gmail.com] Sent: Tuesday, March 25, 2014 3:45 PM To: Jun Fan Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] apache setting On Monday, March 24, 2014, Jun Fan <j.fan@qmul.ac.uk<mailto:j.fan@qmul.ac.uk>> wrote: RewriteEngine on RewriteRule ^(.*) http://localhost:8080$1 [P] I tried to add them to 1) the main conf file: apache2.conf, 2) newly generated httpd.conf by myself under /etc/apache2 folder and 3) newly generated .htaccess file under /etc/apache2/conf.d folder, and none of them gave me the luck. This thread might help you: http://dev.list.galaxyproject.org/Running-Galaxy-behind-apache2-td4624545.ht... --Carlos