Hi guys, another error appeared after we installed NCBI BLAST + to our local Galaxy instance and wanted to test it. After uploading some testdata to our history we tried to run blastn on it and got the following error: Fatal error: Matched on Error: Error: NCBI C++ Exception: "/opt/galaxy/galaxy-dist/database/tmp/tmphx2DHp/ncbi-blast-2.2.26+-src/c++/src/corelib/ncbiobj.cpp", line 689: Critical: ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL pointer. With some testing around we figure out the problem: ./blastn -query ~/test.fasta -db nt -task blastn -evalue 0.001 -out ~/out2.txt -outfmt "6 std sallseqid score nident positive gaps ppos qframe sframe qseq sseq qlen slen" -num_threads 8 Doesn't work until we replaced the "num_threads 8" like it is written in the original "ncbi_blastn_wrapper.xml" with "num_threads 1" Editing the .xml also solved the problem within the galaxy surface. Now my question is, is there any other workaround without editing the" ncbi_blastn_wrapper.xml"? Searching in the documentation and the mailing list, I did not find anything about this. We believe this could be of common interest. It would be great if anybody could comment on this. Thanks a lot, Thomas