Hi, I was trying to upload a 43Gb BAM file to Galaxy when I encountered this problem. I am new to galaxy and python. I have tried uploading files in other formats and they have worked fine. I am using galaxy as a local installation and appreciate any thoughts on how to fix this. Thanks Sukha =========== Traceback (most recent call last): File "/Data/galaxy/galaxy-dist/tools/data_source/upload.py", line 384, in <module> __main__() File "/Data/galaxy/galaxy-dist/tools/data_source/upload.py", line 373, in __main__ add_file( dataset, registry, json_file, output_path ) File "/Data/galaxy/galaxy-dist/tools/data_source/upload.py", line 312, in add_file if link_data_only == 'copy_files' and datatype.dataset_content_needs_grooming( output_path ): File "/Data/galaxy/galaxy-dist/lib/galaxy/datatypes/binary.py", line 113, in dataset_content_needs_grooming version = self._get_samtools_version() File "/Data/galaxy/galaxy-dist/lib/galaxy/datatypes/binary.py", line 97, in _get_samtools_version output = subprocess.Popen( [ 'samtools' ], stderr=subprocess.PIPE, stdout=subprocess.PIPE ).communicate()[1] File "/Data/galaxy/Python-2.7.1/Lib/subprocess.py", line 672, in __init__ errread, errwrite) File "/Data/galaxy/Python-2.7.1/Lib/subprocess.py", line 1202, in _execute_child raise child_exception OSError: [Errno 2] No such file or directory ============== Report this error to the Galaxy Team The Galaxy team regularly reviews errors that occur in the application. However, if you would like to provide additional information (such as what you were trying to do when the error occurred) and a contact e-mail address, we will be better able to investigate your problem and get back to you. ___________________________________________________________