Ofcourse I spoke too soon. I get a problem in that the conversion of my dataset reports that it cannot find the chromosome length files, even though trickster itself has no problem showing it. I looked at the problem script and it is showing below It seems that the script is passed the path to the chromosome files like this "tool-data/shared/ucsc/chrom/hg19-decoy.len" This will clearly never work since this file is not relative to the working directory.. SO somewhere a script forgets to add the ${GALAXY_DATA_DIR} or whatever that parameter is... I could probably hardcode the location of the tool-data directory, but I don't think that should be the solution. Why do I only have this issue? SHOULD I have hardcoded the location of tool-data? The universe_wgi.ini.sample file does not hardcode this. Am I missing some updates? Thanks, Thon #!/bin/sh GALAXY_LIB="None" if [ "$GALAXY_LIB" != "None" ]; then if [ -n "$PYTHONPATH" ]; then PYTHONPATH="$GALAXY_LIB:$PYTHONPATH" else PYTHONPATH="$GALAXY_LIB" fi export PYTHONPATH fi TMPDIR=/mnt/ngs/analysis/svcgalaxy/DATATEST/TMP export TMPDIR cd /mnt/ngs/analysis/svcgalaxy/DATATEST/job_working_directory/001/1916 grep -v '^#' /mnt/ngs/analysis/svcgalaxy/DATATEST/files/002/dataset_2288.dat | sort -k1,1 | bedtools genomecov -bg -split -i stdin -g tool-data/shared/ucsc/chrom/hg19-decoy.len > temp.bg ; bedGraphToBigWig temp.bg tool-data/shared/ucsc/chrom/hg19-decoy.len /mnt/ngs/analysis/svcgalaxy/DATATEST/files/002/dataset_2315.dat; cd /mnt/ngs/analysis/svcgalaxy/galaxy-test; /mnt/ngs/analysis/svcgalaxy/galaxy-test/set_metadata.sh /mnt/ngs/analysis/svcgalaxy/DATATEST/files /mnt/ngs/analysis/svcgalaxy/DATATEST/job_working_directory/001/1916 . /mnt/ngs/analysis/svcgalaxy/galaxy-test/universe_wsgi.ini /mnt/ngs/analysis/svcgalaxy/DATA/TMP/tmp2KAn7W /mnt/ngs/analysis/svcgalaxy/DATATEST/job_working_directory/001/1916/galaxy.j son /mnt/ngs/analysis/svcgalaxy/DATATEST/job_working_directory/001/1916/metadata _in_HistoryDatasetAssociation_2551_XsCZer,/mnt/ngs/analysis/svcgalaxy/DATATE ST/job_working_directory/001/1916/metadata_kwds_HistoryDatasetAssociation_25 51_iNUAr_,/mnt/ngs/analysis/svcgalaxy/DATATEST/job_working_directory/001/191 6/metadata_out_HistoryDatasetAssociation_2551_ZHhosg,/mnt/ngs/analysis/svcga laxy/DATATEST/job_working_directory/001/1916/metadata_results_HistoryDataset Association_2551_tqtiIX,,/mnt/ngs/analysis/svcgalaxy/DATATEST/job_working_di rectory/001/1916/metadata_override_HistoryDatasetAssociation_2551_MOfd8w echo $? > /mnt/ngs/analysis/svcgalaxy/DATATEST/job_working_directory/001/1916/galaxy_1 916.ec Regards, Thon Thon deBoer Ph.D., Bioinformatics Guru California, USA |p: +1 (650) 799-6839 |m: <mailto:thondeboer@me.com> thondeboer@me.com Public profile on <http://www.linkedin.com/pub/thon-de-boer/1/1ba/a5b/> LinkedIn From: galaxy-dev-bounces@lists.bx.psu.edu [mailto:galaxy-dev-bounces@lists.bx.psu.edu] On Behalf Of Thon de Boer Sent: Friday, August 23, 2013 5:47 PM To: 'Jeremy Goecks' Cc: 'Galaxy-dev Galaxy-dev' Subject: Re: [galaxy-dev] Why does my trackster complain about not being able to display BED files? I tracked it down to a problem with the API proxy configuration. I had the API calls diverted to a different server since I wanted to ensure that API calls would be handled by a different server, but that does not seem to work correctly for the API calls used in trickster. Issue resolved. Regards, Thon Thon deBoer Ph.D., Bioinformatics Guru California, USA |p: +1 (650) 799-6839 |m: <mailto:thondeboer@me.com> thondeboer@me.com Public profile on <http://www.linkedin.com/pub/thon-de-boer/1/1ba/a5b/> LinkedIn From: galaxy-dev-bounces@lists.bx.psu.edu <mailto:galaxy-dev-bounces@lists.bx.psu.edu> [mailto:galaxy-dev-bounces@lists.bx.psu.edu] On Behalf Of Thon de Boer Sent: Friday, August 23, 2013 5:12 PM To: 'Jeremy Goecks' Cc: 'Galaxy-dev Galaxy-dev' Subject: Re: [galaxy-dev] Why does my trackster complain about not being able to display BED files? I now have a different problem with a different (production) version of galaxy trickster. I always get this message "Could not load chroms for this dbkey" I have checked that I have files with the [key].len in tool-data/shared/ucsc/chrom and I have twobit files etc. The weird thing is, that the "Create visualization" shows the genome keys I have but this window shows nothing for the dbkey it things it should be getting. So somewhere it "looses" the key. Any ideas? (I'm running the latest update) Regards, Thon Thon deBoer Ph.D., Bioinformatics Guru California, USA |p: +1 (650) 799-6839 |m: <mailto:thondeboer@me.com> thondeboer@me.com Public profile on <http://www.linkedin.com/pub/thon-de-boer/1/1ba/a5b/> LinkedIn From: galaxy-dev-bounces@lists.bx.psu.edu <mailto:galaxy-dev-bounces@lists.bx.psu.edu> [mailto:galaxy-dev-bounces@lists.bx.psu.edu] On Behalf Of Thon de Boer Sent: Friday, August 23, 2013 11:02 AM To: 'Jeremy Goecks' Cc: 'Galaxy-dev Galaxy-dev' Subject: Re: [galaxy-dev] Why does my trackster complain about not being able to display BED files? Thanks Jeremy, I did what you suggested and now it no longer complains! Not sure if I had edited datatypes_conf.xml but just copying the sample worked and let's just hope I did not break any of my changes Regards, Thon Thon deBoer Ph.D., Bioinformatics Guru California, USA |p: +1 (650) 799-6839 |m: <mailto:thondeboer@me.com> thondeboer@me.com Public profile on <http://www.linkedin.com/pub/thon-de-boer/1/1ba/a5b/> LinkedIn From: Jeremy Goecks [mailto:jeremy.goecks@emory.edu] Sent: Thursday, August 22, 2013 11:11 AM To: Anthonius deBoer Cc: sam guerler; Galaxy-dev Galaxy-dev Subject: Re: [galaxy-dev] Why does my trackster complain about not being able to display BED files? Am I missing a converter? Yes, you are missing the bed_to_bigwig converter. If you haven't made changes to your datatypes_conf.xml file, you can just copy datatypes_conf.xml.sample to datatypes_conf.xml to get the needed converters. If you've made changes to datatypes_conf.xml, you'll need to manually add the needed converters. We recently transitioned all the *_to_summary_tree converters to *_to_bigwig, so you'll want to remove the summary_tree converters and replace them with the bigwig converters. And why would a BED file even needed to be converted? Converter = indexer for visualizations. Datasets are indexed so that (a) aggregate (coverage) data is readily available when viewing a large region and (b) finding features in a small region (when zoomed in) is fast. J.
