Thanks a lot! I got it to work exactly as you said. Cheers, Christopher On Sep 18, 2018, at 15:56, Marius van den Beek <m.vandenbeek@gmail.com<mailto:m.vandenbeek@gmail.com>> wrote: Hi Christopher, due to the old version of Galaxy you need to manually update Conda (conda update conda) and explicitly set the current channel order in your galaxy.ini file, this should be conda_ensure_channels = iuc,conda-forge,bioconda,defaults With these set you can remove the exisiting conda environment for deseq2 (conda env remove -n mulled-v1-dbc1e05d0db06d983572e7eca0df68af225d8f398b6e11dd4d586075518c4101) and then re-install deseq2. Once you update to a newer Galaxy version you can comment the conda_ensure_channels setting again and continue using the default channel order that Galaxy ships. Hope that helps, Marius On Tue, 18 Sep 2018 at 12:18, Christopher Previti <christopher.previti@dkfz-heidelberg.de<mailto:christopher.previti@dkfz-heidelberg.de>> wrote: Dear all, I have been trying to get deseq2 (latest version 17, 2018-09-05 from iuc; we are still running on galaxy version 17.09) to work properly but to no avail. Older versions have other errors, I have focused on the latest version. I get this cryptic error when I run it: /opt/galaxy/galaxy/database/dependencies/_conda/envs/mulled-v1-dbc1e05d0db06d983572e7eca0df68af225d8f398b6e11dd4d586075518c4101/lib/R/bin/exec/R: symbol lookup error: /opt/galaxy/galaxy/database/dependencies/_conda/envs/mulled-v1-dbc1e05d0db06d983572e7eca0df68af225d8f398b6e11dd4d586075518c4101/lib/R/bin/exec/../../lib/../../libreadline.so.6: undefined symbol: PC /opt/galaxy/galaxy/database/dependencies/_conda/envs/mulled-v1-dbc1e05d0db06d983572e7eca0df68af225d8f398b6e11dd4d586075518c4101/lib/R/bin/exec/R: symbol lookup error: /opt/galaxy/galaxy/database/dependencies/_conda/envs/mulled-v1-dbc1e05d0db06d983572e7eca0df68af225d8f398b6e11dd4d586075518c4101/lib/R/bin/exec/../../lib/../../libreadline.so.6: undefined symbol: PC Does anybody know what to do about this? Thanks and best regards, Christopher -- Dr. Christopher Previti Genomics and Proteomics Core Facility High Throughput Sequencing (W190) Bioinformatician German Cancer Research Center (DKFZ) Foundation under Public Law Im Neuenheimer Feld 580 69120 Heidelberg Germany Room: B2.102 (INF580/TP3) Phone: +49 6221 42-4661 christopher.previti@dkfz.de<http://www.dkfz.de/> www.dkfz.de<http://www.dkfz.de/> Management Board: Prof. Dr. Michael Baumann, Prof. Dr. Josef Puchta VAT-ID No.: DE143293537 Vertraulichkeitshinweis: Diese Nachricht ist ausschlie?lich f?r die Personen bestimmt, an die sie adressiert ist. Sie kann vertrauliche und/oder nur f?r den/die Empf?nger bestimmte Informationen enthalten. Sollten Sie nicht der bestimmungsgem??e Empf?nger sein, kontaktieren Sie bitte den Absender und l?schen Sie die Mitteilung. Jegliche unbefugte Verwendung der Informationen in dieser Nachricht ist untersagt. ___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: https://lists.galaxyproject.org/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/