Hi Brad,

They're binary BAM files. I followed the section below, but perhaps I missed something. Thanks for the clarification on the Paste server, and I think I'll try nginx next if I can't get forwarding through Apache to work.

Cheers,

-Kip

On Thu, Oct 13, 2011 at 1:17 PM, Brad Chapman <chapmanb@50mail.com> wrote:

Kip;
Are these binary BAM or BigWig type files you are having trouble
displaying at UCSC? I don't believe the Paste server has support for the
byte-range requests that UCSC needs, and you need to send files through
the proxy server. I've done this with nginx so don't have direct Apache
experience here, but the 'Sending files using Apache' section here
should get it working:

http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy

Brad


> Perhaps this is a distribution issue. For those who have gotten the "Display
> at UCSC" link to work on a local server or workstation, what linux
> distribution and version did you use? Has anyone used this successfully with
> Red Hat Enterprise Linux 6.1?
>
> Best,
>
> -Kip
>
> On Fri, Oct 7, 2011 at 6:28 PM, Bodi, Kip <Kip.Bodi@tufts.edu> wrote:
>
> > Hi all,
> >
> > I have a local galaxy instance, proxied by Apache, and am trying to get the
> > 'display at UCSC' link to work. I've followed all of the instructions and am
> > nearly there, but now I'm getting this response from UCSC when it tries to
> > load the track:
> >
> > "Byte-range request was ignored by server. Expected Partial Content 206."
> > etc.
> >
> > I think the error is because the PasteWSGIServer isn't allowing byte-range
> > requests. When I look at the header, "Accept-Ranges: bytes" is not present:
> > [galaxy@galaxy galaxy_dist]$ curl --head localhost
> > HTTP/1.1 200 OK
> > Date: Fri, 07 Oct 2011 22:23:49 GMT
> > Server: PasteWSGIServer/0.5 Python/2.6.6
> > content-type: text/html; charset=UTF-8
> > Set-Cookie:
> > galaxysession=c6ca0ddb55be603a4e98b4f13a4db8bfb8635f0aa576697114d40b13a8355efe2cf7a075ef81d769;
> > expires=Thu, 05-Jan-2012 17:23:49 GMT; Max-Age=7776000; Path=/; Version=1
> > Vary: Accept-Encoding
> > Connection: close
> >
> > Whereas on another server, "Accept-Ranges: bytes" appears in the header:
> > [galaxy@galaxy galaxy_dist]$ curl --head apollo.med.tufts.edu<
> > http://apollo.med.tufts.edu>
> > HTTP/1.1 403 Forbidden
> > Date: Fri, 07 Oct 2011 22:22:42 GMT
> > Server: Apache/2.2.21 (Fedora)
> > Accept-Ranges: bytes
> > Content-Length: 4609
> > Connection: close
> > Content-Type: text/html; charset=UTF-8
> >
> > I'm running RHEL6 with the latest version of galaxy-dist. I've tried
> > manually enabling 'accept-ranges' but haven't had any luck. Any suggestions?
> > Can I enable it somehow in universe_wsgi.ini?
> >
> > Best,
> >
> > -Kip



--
Kip Lord Bodi Jr.
Tufts University School of Medicine
136 Harrison Avenue, South Cove 502
Boston, MA 02118
617-636-3763
kip.bodi@tufts.edu
www.tucf.org | genomics.med.tufts.edu