Hello everyone,
I am comparing two samples (control and treated) paired end RNA Seq data. In the cuffdiff output I have noticed that few genes have zero FPKM value in one sample and other sample has significant FPKM value.
I want to identify uniquely expressed genes identified only in one sample (either control or treated).
My question: If something is measured with significance in one
sample (high FPKM), but not measured at all in another sample (Zero FPKM), should I consider that gene as significant?
Can anyone explain this.
Thanks
suz