I have a question about sending a bed to Ensembl or bam to IGV through Galaxy.

I have setup a galaxy on my own pccluster.

Everything is fine.

But when I want to display the bam file to IGV, I got the error message about error loading files.

“Error loading http://xxx.xx.xxx/galaxy/display_application/xxxxxxxxxx/data/galaxy_xxxxx.bam: An error occurred while accessing: http://xxx.xx.xxx/galaxy/display_application/xxxxxxxxxx/data/galaxy_xxxxx.bam Read error; BinaryCode in readmode; streamed file (filename not available)”

When I use the url to get the file directly, it can be successfully downloaded.

I meet the same situation when I send the bed file to display at Ensembl.

The tract will not be shown on Ensembl.

When I want to send the bed manually through url, I also get the error message about loading files.

“Unable to access file. Server response: 500 read timeout”

Does anyone know how to solve the problem??

Thanks a lot!!

Best Regards,

Terry Yeh