Hi Nate, So I went ahead and set `static_enabled = False` in universe_wsgi.ini, restarted uWSGI, and browsed to localhost. I'm including the results of each step below: After restarting uWSGI, the last few lines of uwsgi.log looked like this: WSGI app 0 (mountpoint='') ready in 11 seconds on interpreter 0xa934c0 pid: 3561 (default app) *** uWSGI is running in multiple interpreter mode *** spawned uWSGI master process (pid: 3561) spawned uWSGI worker 1 (pid: 3575, cores: 4) spawned uWSGI worker 2 (pid: 3576, cores: 4) spawned uWSGI worker 3 (pid: 3583, cores: 4) spawned uWSGI worker 4 (pid: 3587, cores: 4) spawned uWSGI worker 5 (pid: 3591, cores: 4) spawned uWSGI worker 6 (pid: 3595, cores: 4) spawned uWSGI worker 7 (pid: 3599, cores: 4) spawned uWSGI worker 8 (pid: 3603, cores: 4) spawned uWSGI worker 9 (pid: 3607, cores: 4) spawned uWSGI worker 10 (pid: 3611, cores: 4) *** Stats server enabled on 127.0.0.1:9191 fd: 30 *** ...so no traceback this time! Then, browsing to localhost redirects to https://localhost and finally returns: "Internal Server Error Galaxy was unable to successfully complete your request An error occurred. This may be an intermittent problem due to load or other unpredictable factors, reloading the page may address the problem. The error has been logged to our team." That request was logged to uwsgi.log: 127.0.0.1 - - [03/Feb/2015:14:51:01 -0400] "GET / HTTP/1.1" 500 - "-" "Mozilla/5.0 (X11; Ubuntu; Linux x86_64; rv:35.0) Gecko/20100101 Firefox/35.0" Error - <type 'exceptions.KeyError'>: 'SCRIPT_NAME' URL: https://localhost/ File 'lib/galaxy/web/framework/middleware/error.py', line 149 in __call__ app_iter = self.application(environ, sr_checker) File '/home/galaxy/galaxy-dist/eggs/Paste-1.7.5.1-py2.7.egg/paste/recursive.py', line 84 in __call__ return self.application(environ, start_response) File '/home/galaxy/galaxy-dist/eggs/Paste-1.7.5.1-py2.7.egg/paste/httpexceptions.py', line 633 in __call__ return self.application(environ, start_response) File 'lib/galaxy/web/framework/base.py', line 132 in __call__ return self.handle_request( environ, start_response ) File 'lib/galaxy/web/framework/base.py', line 159 in handle_request trans = self.transaction_factory( environ ) File 'lib/galaxy/web/framework/webapp.py', line 71 in <lambda> self.set_transaction_factory( lambda e: self.transaction_chooser( e, galaxy_app, session_cookie ) ) File 'lib/galaxy/web/framework/webapp.py', line 102 in transaction_chooser return GalaxyWebTransaction( environ, galaxy_app, self, session_cookie ) File 'lib/galaxy/web/framework/webapp.py', line 207 in __init__ self._ensure_logged_in_user( environ, session_cookie ) File 'lib/galaxy/web/framework/webapp.py', line 441 in _ensure_logged_in_user if self.request.path.startswith( external_display_path ): File 'lib/galaxy/web/framework/base.py', line 258 in __get__ value = self.func( obj ) File 'lib/galaxy/web/framework/base.py', line 341 in path return self.environ['SCRIPT_NAME'] + self.environ['PATH_INFO'] KeyError: 'SCRIPT_NAME' CGI Variables ------------- DOCUMENT_ROOT: '/usr/share/nginx/html' HTTP_ACCEPT: 'text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8' HTTP_ACCEPT_ENCODING: 'gzip, deflate' HTTP_ACCEPT_LANGUAGE: 'en-US,en;q=0.5' HTTP_CONNECTION: 'keep-alive' HTTP_HOST: 'localhost' HTTP_USER_AGENT: 'Mozilla/5.0 (X11; Ubuntu; Linux x86_64; rv:35.0) Gecko/20100101 Firefox/35.0' PATH_INFO: '/' REMOTE_ADDR: '127.0.0.1' REMOTE_PORT: '50064' REQUEST_METHOD: 'GET' REQUEST_URI: '/' SERVER_NAME: 'localhost' SERVER_PORT: '443' SERVER_PROTOCOL: 'HTTP/1.1' UWSGI_SCHEME: 'https' WSGI Variables -------------- application: <paste.recursive.RecursiveMiddleware object at 0x7f2ec2f32290> is_api_request: False paste.cookies: (<SimpleCookie: >, '') paste.expected_exceptions: [<class 'paste.httpexceptions.HTTPException'>] paste.httpexceptions: <paste.httpexceptions.HTTPExceptionHandler object at 0x7f2ec2f32210> paste.recursive.forward: <paste.recursive.Forwarder from /> paste.recursive.include: <paste.recursive.Includer from /> paste.recursive.include_app_iter: <paste.recursive.IncluderAppIter from /> paste.recursive.script_name: '' paste.throw_errors: True request_id: 'fadecee2abdd11e4bfb614feb5e02dd6' uwsgi.core: 0 uwsgi.node: 'i changed this string to hide the server name' uwsgi.version: '2.0.9' wsgi process: 'Multi process AND threads (?)' wsgi.file_wrapper: <built-in function uwsgi_sendfile> ------------------------------------------------------------ [pid: 3607|app: 0|req: 1/1] 127.0.0.1 () {40 vars in 593 bytes} [Tue Feb 3 14:51:01 2015] GET / => generated 777 bytes in 230 msecs (HTTP/1.1 500) 1 headers in 63 bytes (1 switches on core 0) I thought I would include some pieces of universe_wsgi.ini to help with troubleshooting: # ---- HTTP Server ---------------------------------------------------------- # Configuration of the internal HTTP server. [server:main] # The internal HTTP server to use. Currently only Paste is provided. This # option is required. use = egg:Paste#http # The port on which to listen. #port = 8080 # The address on which to listen. By default, only listen to localhost (Galaxy # will not be accessible over the network). Use '0.0.0.0' to listen on all # available network interfaces. host = 0.0.0.0 # Use a threadpool for the web server instead of creating a thread for each # request. use_threadpool = True # Number of threads in the web server thread pool. threadpool_workers = 10 # Set the number of seconds a thread can work before you should kill it (assuming it will never finish) to 3 hours. threadpool_kill_thread_limit = 10800 # ---- uwsgi ---------------------------------------------------------- [uwsgi] processes = 10 stats = 127.0.0.1:9191 socket = 127.0.0.1:4001 pythonpath = lib threads = 4 logto = /home/galaxy/galaxy-dist/uwsgi.log master = True Also, we are using a self-signed certificate as a temporary solution, so not sure if that matters. Thanks!