Hi, First, Sorry but, I'm a newbie with Apache and my questions will certainly appear stupid. To configure apache2, I've followed this wiki : http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy And I write these instructions : <Proxy http://localhost:8080> Order deny,allow Allow from all </Proxy> RewriteEngine on <Location "/"> # Define the authentication method AuthType Basic AuthName Galaxy AuthUserFile /home/nate/htpasswd Require valid-user # Take the $REMOTE_USER environment variable and set it as a header in the proxy request. RewriteCond %{IS_SUBREQ} ^false$ RewriteCond %{LA-U:REMOTE_USER} (.+) RewriteRule . - [E=RU:%1] RequestHeader set REMOTE_USER %{RU}e </Location> RewriteRule ^/static/style/(.*) /home/nate/galaxy-dist/static/june_2007_style/blue/$1 [L] RewriteRule ^/static/(.*) /home/nate/galaxy-dist/static/$1 [L] RewriteRule ^/favicon.ico /home/nate/galaxy-dist/static/favicon.ico [L] RewriteRule ^/robots.txt /home/nate/galaxy-dist/static/robots.txt [L] RewriteRule ^(.*) http://localhost:8080$1 [P] </Location> But several problems appeared when I launched my Galaxy. First, must this instructions block be in the file apache2.conf or in the file default of sites-available directory ? If it must be in the file default, is it between the <VirtualHost> tags or out of these tags ? Second, I've created an htpaswd file in my home directory, but when I launch galaxy, it doesn't request me a login and password but it says me that Galaxy requires an user authentification and it must be an error in my apache configuration. Is it a problem with the instructions or with their place in configuration file ? Third the line </Location> appears two times in your instructions but it's wrong for apache. I've deleted the first but I'm note sure that Rewrite instructions must be in the <Location> block. And finally, I want to work with severals web processes and I want to add this configuration lines : <Proxy balancer://galaxy> ... </Proxy> Must I add to previous lines and where ? or Must I replace certain lines of previous block and if yes : which lines ? Thank you very much. Julie