Hi Enis Afgan,
Thank you very much for you answer.
I have some questions abount the tool of Megablast in NGS:Mapping when I download the needed indexes .
,nt 28-Jan-2013,wgs 28-Jan-2013,phiX174;but there are too many types of the indexes file in ftp://ftp.ncbi.nlm.nih.gov/blast/db/ ,for example : nt.00.tar.gz,nt.01.tar.gz,nt.02.tar.gz....I don't know the differents among these files ,and I don't which one  I should choose to download.In addition ,I can't find the index file for phiX174.
Could you help me and give me some advice please? Thanks.
 
shenwiyn
 
From: Enis Afgan
Date: 2013-03-05 10:38
To: shenwiyn
CC: Rafael Hernández; galaxy-dev@bx.psu.edu
Subject: Re: Tools and Data
Correct; certain NSG tools require additional configuration for their indices (http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup)


On Tue, Mar 5, 2013 at 1:07 PM, shenwiyn <shenwiyn@gmail.com> wrote:
Hi,
I've installed Galaxy in local as what Rafael does.I want to make sure that the basic functions work successfully just depending on the necessary tools in Tool dependencies manual,and the NGS tools not only depend on the necessary tools but also some another needed data(such as indexes,and so on).
 
thakks
shenwy