Hello, I'm trying to install galaxy on a apache server,and i have the same error message than David HOOVER when i tried to generate the authentification files. Thanks to David Hoover who gives me some helpful explanation. When i read the thread on gmod, it seems,that the problem is solved (http://gmod.827538.n3.nabble.com/trouble-with-authentication-through-Apache-... <http://gmod.827538.n3.nabble.com/trouble-with-authentication-through-Apache-td1809681.html>) but I did not find the solution in my case. I use LDAP authentification and Galaxy is implemented on a VM. Could you please explain me how to solve it. I really need help. I modified universe file ( in galaxy-dist) and i created .htaccess file in galaxy-dist/ : * in universe_wsgi.ini: use_remote_user = True remote_user_maildomain = example.org <http://example.org> * in .htaccess file in galaxy-dist/ Directory : 1 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_1> # Define Galaxy as a valid Proxy 2 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_2> <Proxy http://localhost:8080> 3 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_3> Order deny,allow 4 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_4> Allow from all 5 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_5> </Proxy> 6 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_6> #!highlight apache 7 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_7> <Location "/"> 8 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_8> AuthType Basic 9 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_9> AuthBasicProvider *ldap* 10 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_10> Auth*LDAP*URL "*ldap*://server:389/ou=People,dc=example,dc=org?uid?sub?(objectClass=person)" 11 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_11> Authz*LDAP*Authoritative off 12 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_12> Require valid-user 13 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_13> # Set the REMOTE_USER header to the contents of the *LDAP* query response's "uid" attribute 14 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_14> RequestHeader set REMOTE_USER %{AUTHENTICATE_uid}e 15 <http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy?highlight=%28Ldap%29#CA-e07a9dbbf4f816c0970d4995f4abb957a6683638_15> </Location> with location, AuthBasicProvider and proxy competed But i have the error : Access to Galaxy is denied Galaxy is configured to authenticate users via an external method (such as HTTP authentication in Apache), but a username was not provided by the upstream (proxy) server. This is generally due to a misconfiguration in the upstream server. Thank you, and best regards Sarah Maman