Hello, I want to know if I can do something on galaxy. I have a folder for the chromosome 1 with start of G4 and start of ORI. For each start G4 I associated all of start ORI. For example if I have 2 start G4 (2 lines) and 4 start ORI (2 lines), I will have on a new file 8 lines. startG4-1 start ORI-1 startGA-1 startORI-2 startG4-1 startORI-3 startG4-1 startORI-4 startG4-2 startORI-1 startG4-2 starORI-2 startG4-2 startORI-3 startG4-2 startORI-4 Now I do the difference between the first column and the second and I want the absolute value of this difference to have the distance between the start G4 and the start ORI. I obtain a third column. Now that I want is the minimum of those absolute values. And I want to know which start G4 and which start ORI who correspond to this mimnimum. For example if the minimum of startG4-1 is on the third column and the minimum of startG4-2 is on the second column. I want as result startG4-1 startORI-3 min(c2-c1) startG4-2 startORI-2 min(c2-c1) So know my question is, do you know if I can obtain that I wnt on Galaxy, and if YES how? Best regards, Victoria Spartacus