Hi Joan,

For IGV and most external displays that work on BAM and other larger seekable file formats (bigwig, bigbed, etc.) you'll need to use and configure a proxy server for your local Galaxy instance that provides byte-range request capabilities, such as nginx (http://wiki.galaxyproject.org/Admin/Config/Performance/nginx%20Proxy) or apache (http://wiki.galaxyproject.org/Admin/Config/Apache%20Proxy). 


Thanks for using Galaxy,

Dan


On Nov 26, 2012, at 6:11 AM, <PROTASIO@clinic.ub.es> <PROTASIO@clinic.ub.es> wrote:

Hi all,
 
I’m having  problems when I click to visualize one BAM file from galaxy to IGV version 2.1.24. I’ve tried the local and also the web display options  but I have the same problem. I have tried also to download first the bam file from galaxy and then loading into IGV manually and it works. I also have tried to modify the .jnlp generated but it does not work.
 
IGV shows the next error message:
 
ERROR [2012-11-26 11:44:54,591]  [IGV.java:1555] [pool-1-thread-1]  net.sf.samtools.util.RuntimeEOFException: Premature EOF; BinaryCodec in readmode; streamed file (filename not available)
ERROR [2012-11-26 11:44:54,592]  [IGV.java:1494] [pool-1-thread-1]  Error loading tracks
org.broad.igv.exceptions.DataLoadException: An error occurred while accessing:   http://172.21.104.21:8080/display_application/9e23127bd2c9a880/igv_bam/web_link_main/8d9bcc17f9fede63/data/galaxy_9e23127bd2c9a880.bam<br>Premature EOF; BinaryCodec in readmode; streamed file (filename not available)
 
Thanks for your help.
 
 
Joan Protasio,  Bioinformatician & Software Engineer
Bioinformatics Platform 
August Pi i Sunyer Biomedical Research Institute (IDIBAPS)
C/ Rosselló 153, Barcelona
http://bioinformatics.fcrb.es
+34  93 227 54 00 ext. 4522
 
___________________________________________________________
Please keep all replies on the list by using "reply all"
in your mail client.  To manage your subscriptions to this
and other Galaxy lists, please use the interface at:

 http://lists.bx.psu.edu/