So I just want to install one target database so that I can simply test whether blast is installed and working correctly. Are there any included with Galaxy, or do I need to download a database? Could you guys point me to a good file to test with? Following the directions you sent I got to the point of downloading a file. I tried download ftp://ftp.ncbi.nlm.nih.gov/blast/db/nr.00.tar.gz but I don't see a *.chunk file nor *.nhr, nin, nsq etc that are referenced in the included blastdb.loc Thanks, Greg On Mon, Dec 10, 2012 at 3:35 PM, Jeremy Goecks <jeremy.goecks@emory.edu> wrote:
Please see the Megablast section on this page for instructions about getting and adding target databases to Galaxy:
http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup
Best, J.
On Dec 10, 2012, at 2:59 PM, greg wrote:
I have a fresh install on our cluster.
I'm trying to test out the Megablast tool. The problem is that nothing is listed under "against target database". Where do I get the target databases and how do I tell galaxy about them?
Thanks,
Greg
Here's what the tool looks like:
Megablast (version 1.2.0) Compare these sequences: against target database: using word size: Size of best perfect match (-word_size) report hits above this identity (-perc_identity): no cutoff if 0 set expectation value cutoff (-evalue): Filter out low complexity regions? (-dust): ___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: