I have seen this error many times (for various mako.py files) and it is often either when the user running it has insufficient privileges or the file permissions on a folder (could be several directories upstream) or a file have changed in some way. If you do a &#39;ps -ef | grep python&#39; do you see the paster.py process running with your expected user? Do you use sudo to elevate the privileges when you run it? <br>
<br>I use a &#39;galaxy&#39; user but I execute run.sh (which I have created a service script for in /etc/init.d) with sudo while logged in as galaxy. Also you might want to do an &#39;ls -l&#39; in the compiled_templates/ directory and look at the file permissions and file ownership. <div>
<br></div><div>-Josh</div>