Hi,
I just installed Galaxy in local Centos Vm. I did my best to get everything working ( I think). I followed the instructions in this Wiki (http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup) to install reference genomes for the tools
Bwa and Bowtie. Here is what I did in details:
1.
I installed all the software and added them to the PATH and everything seems to be working.
2.
Then I created the indices as explained.
3.
I put all the indices in the same recommended directory structure and gave all access rights to the Galaxy user which runs the Galaxy server.
4.
I created the loc files (bowtie_indices.loc and bowtie_indices_color.loc and bwa_index.loc) and populate them with the recommended contents) and place them in ~/tool_data/ folder.
5.
I copied the xml and py files from this location and put them into ~/tools/sr_mapping/….
https://bitbucket.org/galaxy/galaxy-dist/src/da9d740fce31/tools/sr_mapping This step was not mentioned in the instruction but I thought this might be the cause.
6.
I restarted the server.
Once I select NGS:Mapping tool and Bwa or Bowtie I do not see the reference genome in the list. Can someone tell me what I missed or how to go about debugging it?
I appreciate any kind of help….