Hi Greg, I will be at the galaxy conference and am experiencing the same problem. I would love to meet with you to look at this together. Emily From: Greg Von Kuster [mailto:greg@bx.psu.edu] Sent: Wednesday, July 11, 2012 7:55 AM To: Luobin Yang Cc: TerAvest, Emily; galaxy-dev@bx.psu.edu Subject: Re: [galaxy-dev] Sample tracking data transfer hangs "in queue" forever Hello Luobin, I'm on my way to ISMB for a week, and then will be at the Galaxy Community Conference the following week, so I won't have time to help you track this problem down until some time in August unless you will be at either of these 2 conferences. Sorry for the inconvenience on this, but my schedule over the next few weeks is very hectic. If you'll be at either ISMB or the GCC, we can certainly get together to look at this. Greg Von Kuster On Jul 9, 2012, at 1:24 PM, Luobin Yang wrote: Hi, Greg, I've still got issues after I downloaded the latest version from the dist repository. So after I selected the datasets that I would like to transfer from the sequencer and click the "Transfer" button, Galaxy generates an error message : "Invalid sample id (None)"... I tried to delete this dataset using the "manage datasets" menu, and Galaxy generates the same error message: "Invalid sample id (None)". It seems galaxy system generates the sample id automatically (the first one is sample_1), not sure why this happens... Luobin On Fri, Mar 30, 2012 at 8:30 AM, Greg Von Kuster <greg@bx.psu.edu<mailto:greg@bx.psu.edu>> wrote: Hello Emily, This issue should be resolved in change set 6924:12b14f3e78e9, which is currently only available from our central repository. It will not be available in the dist repository fro some time, so you'll have to pull it from Galaxy central (https://bitbucket.org/galaxy/galaxy-central) if you want it now. Thanks very much for reporting this problem, and we apologize for the inconvenience it caused. Greg Von Kuster On Mar 29, 2012, at 1:49 PM, TerAvest, Emily wrote:
Hi Leandro,
I am also experiencing the same problem with the latest version of galaxy. I just attempted to connect our sequencer for the first time yesterday. I do not have an older version of galaxy to test and compare to see if it works in earlier versions. I am able to transfer data from the sequence to the import directory, however it is not moved to the data library. My data_transfer.log also has the same error. "The server could not comply with the request since it is either malformed or otherwise incorrect."
Does anyone have a solution for this?
Thanks Emily
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Message: 17 Date: Wed, 28 Mar 2012 13:15:47 +0200 From: Leandro Hermida <softdev@leandrohermida.com<mailto:softdev@leandrohermida.com>> To: Luobin Yang <yangluob@isu.edu<mailto:yangluob@isu.edu>> Cc: Galaxy Dev <galaxy-dev@bx.psu.edu<mailto:galaxy-dev@bx.psu.edu>> Subject: Re: [galaxy-dev] Sample tracking data transfer hangs "in queue" forever Message-ID: <CAOhzmPJ67udRQBhfChgyxNrXsm3QcY1c7jna1kR0aJBNKEKJxA@mail.gmail.com<mailto:CAOhzmPJ67udRQBhfChgyxNrXsm3QcY1c7jna1kR0aJBNKEKJxA@mail.gmail.com>> Content-Type: text/plain; charset=ISO-8859-1
Dear Galaxy Dev,
Ok I have gotten further found out what was going on, in my data_transfer.log I was getting HTTP 404 Not Authorized when the data transfer was trying to access Galaxy API URLs http://galaxyserver/api/.<http://galaxyserver/api/>.. This is because we are using external user authentication as documented in http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy and this puts all of Galaxy behind this authentication. Since the Galaxy API uses API keys to essentially authenticate you have to change you <Location /> ... </Location> container to not match URLs starting with /api. To do this you have to change it to <LocationMatch "^/(?!api)"> .... </LocationMatch>.
best, leandro
On Mon, Mar 26, 2012 at 6:10 PM, Luobin Yang <yangluob@isu.edu<mailto:yangluob@isu.edu>> wrote:
I've got the same problem and when I looked at the data_transfer.log, I saw the following message:
2012-03-01 15:12:27,338 - datatx_13870 - (u'9c17d84742cd2acb63d88b5bd41d968f', u'http://xxx.xxx.xxx.xxx/api/samples/2d9035b3fc152403', {'sample_dataset_ids': ['a799d38679e985db', '33b43b4e7093c91f'], 'error_msg': '', 'update_type': 'sample_dataset_transfer_status', 'new_status': 'Adding to data library'}) 2012-03-01 15:12:27,342 - datatx_13870 - Error. <!DOCTYPE HTML PUBLIC "-//IETF//DTD HTML 2.0//EN"> <html><head> <title>405 Method Not Allowed</title> </head><body> <h1>Method Not Allowed</h1> <p>The requested method PUT is not allowed for the URL /api/samples/2d9035b3fc152403.</p> <hr>
Using Galaxy instead of Apache as the web server changed the sample status from "in queue" to complete but didn't add the downloaded files to the data library.
Luobin
On Mon, Mar 26, 2012 at 9:54 AM, Leandro Hermida <softdev@leandrohermida.com<mailto:softdev@leandrohermida.com>> wrote:
Dear Galaxy Dev,
I've set up the Galaxy sample tracking system data transfer functionality exactly as specified here https://main.g2.bx.psu.edu/u/rkchak/p/data-transfer, but when I attempt to transfer datasets it puts them into the queue and then never seems to transfer anything.
The galaxy_listener.log shows:
2012-03-26 17:16:24,515 - GalaxyAMQP - GALAXY LISTENER PID: 8738 - {'config_file': 'universe_wsgi.ini', 'http_server_section': 'server:main'} 2012-03-26 17:16:24,518 - GalaxyAMQP - {'exchange': 'galaxy_exchange', 'rabbitmqctl_path':
'/gpfshpc/software/pmrdcdb/galaxy/rabbitmq/rabbitmq_server-2.8.1/sbin/rabbitmqctl', 'userid': 'galaxy_dev', 'routing_key': 'bar_code_scanner', 'queue': 'galaxy_queue', 'host': '127.0.0.1', 'virtual_host': 'galaxy_dev_messaging_engine', 'password': 'mypassword', 'port': '5672'} 2012-03-26 17:16:24,565 - GalaxyAMQP - Connected to rabbitmq server - 127.0.0.1:5672<http://127.0.0.1:5672/> 2012-03-26 17:31:23,393 - GalaxyAMQP - MESSAGE RECVD: data_transfer 2012-03-26 17:31:23,393 - GalaxyAMQP - DATA TRANSFER 2012-03-26 17:31:23,401 - GalaxyAMQP - Started process (25406): python
"/gpfshpc/software/pmrdcdb/galaxy/galaxy_dist_dev/scripts/galaxy_messaging/server/data_transfer.py" " <data_transfer> <galaxy_host>rd-galaxydev.app.pmi</galaxy_host> <api_key>69bbd1a9b18eda2a888e9963820d8ef0</api_key> <data_host>chp723</data_host> <data_user>galaxy</data_user> <data_password>mypassword</data_password> <request_id>12</request_id> <sample_id>12</sample_id> <library_id>15</library_id> <folder_id>52</folder_id> ? ? ? ? ? ? ? ? ? ?<dataset> ?<dataset_id>10</dataset_id> <name>ttt_2.fastq.gz</name> <file>/gpfshpc/data/pmrdcdb/RNA-Seq/datasets/ttt-2.fastq.gz</file> ? ? ? ? ?</dataset><dataset> <dataset_id>9</dataset_id> <name>ttt_1.fastq.gz</name> <file>/gpfshpc/data/pmrdcdb/RNA-Seq/datasets/ttt-1.fastq.gz</file> ? ? ? ? ?</dataset> ? ? ? ? ? ? ? ?</data_transfer>" "universe_wsgi.ini"
The FASTQ files are for testing and aren't very big (7.6 MB), what could be going wrong?
regards, Leandro ___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. ?To manage your subscriptions to this and other Galaxy lists, please use the interface at:
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