Dear Nate,

We have a new issue to use Galaxy. When we login use a general user account, we found:

(1) When we upload six fastq files via URL, three of them can be uploaded successfully while the other three can't be uploaded.

(2) What's more, even those files can be uploaded, we can't perform the mapping with BWA (NGS: Mapping/Map with BWA) either single end or paired-end. The problem is we can't select which file to do the mapping.

(3) If we use an admin account, we can find one file when we perform the mapping and are trying uploading other one to see if we can select from the two files list.

(4) Could you please let me know if Galaxy has some restriction for files number, a single file size, or total files size loaded by one user(account)?

Please find the attached screenshoots for your reference.

Looking forward to hearing from you.

Best Wishes,

Yan

On Tue, Feb 15, 2011 at 9:08 AM, Nate Coraor <nate@bx.psu.edu> wrote:
Yan Luo wrote:
> Dear Nate,
>
> Yes, you are right, for some reason, we couldn't cd to the folder. It is
> fixed and I restart the Galaxy. The only thing is that it needs about 10
> minutes to start. I remembered it is a little fast last week. Is there any
> problem? Anyway, Galaxy works.

Assuming nothing else has changed, it sounds like your NFS server is
having some performance problems.

--nate

>
> Thanks,
>
> Yan
>
> On Mon, Feb 14, 2011 at 8:49 AM, Nate Coraor <nate@bx.psu.edu> wrote:
>
> > Yan Luo wrote:
> > > Dear Nate,
> > >
> > > Thanks for your quick response, I got "sever error". and just killed the
> > > process. When I ran activate as follows and can't active it:
> > >
> > >
> > > kangtu@dscbc-compute:~$ .
> > > /home/kangtu/data/tools/galaxy-dist/galaxy_env/bin/activate
> > > bash: /home/kangtu/data/tools/galaxy-dist/galaxy_env/bin/activate: Stale
> > NFS
> > > file handle
> > > kangtu@dscbc-compute:~$
> >
> > It looks like the NFS mount on which Galaxy lives has gone bad, possibly
> > due to an error with the NFS server.  I suggest contacting a local
> > system administrator for assistance.
> >
> > --nate
> >
> > >
> > >
> > > Any suggestion will be appreciate.
> > >
> > > Best Wishes,
> > >
> > > Yan
> > >
> > >
> > >
> > > On Mon, Feb 14, 2011 at 8:43 AM, Nate Coraor <nate@bx.psu.edu> wrote:
> > >
> > > > Yan Luo wrote:
> > > > > Dear Nate,
> > > > >
> > > > > I have a new problem, last week, I restarted the Galaxy, but it
> > worked
> > > > well.
> > > > > This morning, I found I can't start it from browser, but when I check
> > "ps
> > > > > auxwww|grep python", I still find it is running, what is problem?
> > > >
> > > > What happens when you try to connect via a browser?  Is an error
> > message
> > > > displayed?  Connection timeout?
> > > >
> > > > >
> > > > > kangtu   24468  0.3  0.0 961592 104296 pts/30  Sl+  Feb11  13:43
> > python
> > > > > ./scripts/paster.py serve universe_wsgi.ini
> > > >
> > > > If you reconnect to your screen session and take a look at the output
> > > > from the Galaxy server, there may be error message displayed which
> > would
> > > > explain what the problem is.
> > > >
> > > > --nate
> > > >
> > > > >
> > > > >
> > > > > Looking forward to hearing from you.
> > > > >
> > > > > Best Wishes,
> > > > >
> > > > > yan
> > > > >
> > > > > On Fri, Feb 11, 2011 at 11:16 AM, Yan Luo <luomails@gmail.com>
> > wrote:
> > > > >
> > > > > > Dear Nate,
> > > > > >
> > > > > > I just used "screen", and restarted the galaxy, it works. It is
> > > > perfect,
> > > > > > and I also found that some features can't be used before, when I
> > > > restart it,
> > > > > > everything is fine.
> > > > > >
> > > > > > Have a wonderful weekend!
> > > > > >
> > > > > > Best Wishes,
> > > > > >
> > > > > > Yan
> > > > > >
> > > > > >
> > > > > >
> > > >
> >