Dear All, I get the following errors when I try to Convert BAM to BigWig Traceback (most recent call last): File "/shed_tools/toolshed.g2.bx.psu.edu/repos/brad-chapman/bam_to_bigwig/52bcd04ee0d6/bam_to_bigwig/bam_to_bigwig/bam_to_bigwig.py", line 103, in <module> main(*args, **kwargs) File "/shed_tools/toolshed.g2.bx.psu.edu/repos/brad-chapman/bam_to_bigwig/52bcd04ee0d6/bam_to_bigwig/bam_to_bigwig/bam_to_bigwig.py", line 48, in main convert_to_graph(bam_file, split, config, temp_handle) File "/shed_tools/toolshed.g2.bx.psu.edu/repos/brad-chapman/bam_to_bigwig/52bcd04ee0d6/bam_to_bigwig/bam_to_bigwig/bam_to_bigwig.py", line 76, in convert_to_graph subprocess.check_call(cl, stdout=out_handle) File "/usr/lib/python2.7/subprocess.py", line 535, in check_call retcode = call(*popenargs, **kwargs) File "/usr/lib/python2.7/subprocess.py", line 522, in call return Popen(*popenargs, **kwargs).wait() File "/usr/lib/python2.7/subprocess.py", line 710, in __init__ errread, errwrite) File "/usr/lib/python2.7/subprocess.py", line 1327, in _execute_child raise child_exception OSError: [Errno 2] No such file or directory The tool produced the following additional output: Have 93 references Calculating coverage... or Fatal error: Exit code 1 () bedtools: /galaxy-central/tool_deps/ucsc_tools/312/iuc/package_ucsc_tools_312/2d6bafd63401/lib/libstdc++.so.6: version `GLIBCXX_3.4.15' not found (required by bedtools) needLargeMem: trying to allocate 0 bytes (limit: 100000000000) Traceback (most recent call last): File "/shed_tools/toolshed.g2.bx.psu.edu/repos/brad-chapman/bam_to_bigwig/9163e1db4c16/bam_to_bigwig/bam_to_bigwig.py", line 122, in <module> main(*args, **kwargs) File "/shed_tools/toolshed.g2.bx.psu.edu/repos/brad-chapman/bam_to_bigwig/9163e1db4c16/bam_to_bigwig/bam_to_bigwig.py", line 57, in main convert_to_bigwig(temp_file, sizes, config, outfile) File "/shed_tools/toolshed.g2.bx.psu.edu/repos/brad-chapman/bam_to_bigwig/9163e1db4c16/bam_to_bigwig/bam_to_bigwig.py", line 104, in convert_to_bigwig subprocess.check_call(cl) File "/galaxy-central/tool_deps/python/2.7.10/iuc/package_python_2_7_10/0339c4a9b87b/lib/python2.7/subprocess.py", line 540, in check_call raise CalledProcessError(retcode, cmd) subprocess.CalledProcessError: Command '['bedGraphToBigWig', '/tmp/tmp2F67r6', '/export/galaxy-central/database/files/003/dataset_3116-sizes.txt', '/export/galaxy-central/database/files/003/dataset_3116.dat']' returned non-zero exit status 255 The tool produced the following additional output: Have 93 references Calculating coverage... Converting 0 MB graph file to bigwig.. depending what version of the tool i am using. Any idea what this can be? I am using the latest Docker-galaxy-stable version (16.04) but I think this is not related to docker. All tools are updated. Thank you very much for your help, Philipp