Hi, I decided to format my PC and reinstall everything. I have Ubuntu 12.04LTS installed in one 1TB hard drive (/ and swap) and the /home folder installed in a 3TB hard drive. I created a /tmp partition of 110GB and a /tmp partion of 110GB, both in the 1TB HD. Then I mounted /tmp as /tmpfs using fstab. Here is the df -h output: Filesystem Size Used Avail Use% Mounted on /dev/sda2 817G 3.9G 771G 1% / udev 12G 4.0K 12G 1% /dev tmpfs 100G 16K 100G 1% /tmp tmpfs 4.8G 992K 4.8G 1% /run none 5.0M 0 5.0M 0% /run/lock none 12G 148K 12G 1% /run/shm /dev/sdb2 2.6T 99G 2.4T 4% /home /dev/sdc1 1.9T 1.6T 301G 84% /media/My Book Then I try to upload files as administrator on my local instance of galaxy and it fails. Since I even have a swap partition with 100GB and using the /tmp partition I don't know why it is not working. What I notice is that it is using the /tmpfs mounted on /run, which is only 4.8GB. I also tried to change universe.ini to use /tmp as temporary files storage: # Temporary files are stored in this directory. new_file_path = /tmp I can upload files that are smaller than 4.8GB, but not bigger than it. Previously it was working fine on the other Ubuntu version. I was using /tmp as tmpfs, but I don't know how to configure the Galaxy to use the the tmpfs mounted at /tmp and not the tmpfs mounted at /run. I asked similar question before and I was able to fix it on Ubuntu 10.04 LTS, but now Ubuntu have this tmpfs mounted on /run, which is not in fstab. Thank you. Luciano -- *Luciano Cosme* --------------------------------------------- PhD Candidate Texas A&M Entomology Vector Biology Research Group www.lcosme.com 979 845 1885 cosme@tamu.edu ---------------------------------------------