Hi, After several post in an Apache Forum, I've solve any problem of my apache configuration to enable load balancer configuration. It seems work but when I launch my galaxy application, I arrive in this web page : Service temporarily Unavailable And my log apache shows this : [Tue May 29 09:37:32 2012] [error] (111)Connection refused: proxy: HTTP: attempt to connect to 127.0.0.1:8083 (localhost) failed [Tue May 29 09:37:32 2012] [error] ap_proxy_connect_backend disabling worker for (localhost) [Tue May 29 09:37:32 2012] [error] (111)Connection refused: proxy: HTTP: attempt to connect to 127.0.0.1:8082 (localhost) failed [Tue May 29 09:37:32 2012] [error] ap_proxy_connect_backend disabling worker for (localhost) [Tue May 29 09:37:32 2012] [error] (111)Connection refused: proxy: HTTP: attempt to connect to 127.0.0.1:8081 (localhost) failed [Tue May 29 09:37:32 2012] [error] ap_proxy_connect_backend disabling worker for (localhost) [Tue May 29 09:37:32 2012] [error] (111)Connection refused: proxy: HTTP: attempt to connect to 127.0.0.1:8080 (localhost) failed [Tue May 29 09:37:32 2012] [error] ap_proxy_connect_backend disabling worker for (localhost) It means that galaxy doesn't listen the port 8080, 8081 ... I think it's my run.sh which cannot read my universe_wsgi.ini correctly. Because if it could read correctly, in my terminal I could see this type of line : Handling web0 with log file web0.log...Entering daemon mode... Now, Idon't see that! after the command GALAXY_RUN_ALL=1 sh run.sh --daemon, I just see : Entering Daemon mode. So in my view, it's not an Apache problem but a shell script galaxy problem. Somedy has already met this problem ? Have you a solution ? Thank you. Julie