Hi, I'm hoping you can help out with a problem we are experiencing uploading files to our own Galaxy instance. When a user tries to upload a file to Galaxy through their web browser, the job errors out: Traceback (most recent call last): File "/gpfs/fs1/sfw/cibem_galaxy/app/galaxy/tools/data_source/upload.py", line 430, in <module> __main__() File "/gpfs/fs1/sfw/cibem_galaxy/app/galaxy/tools/data_source/upload.py", line 407, in __main__ for line in open( sys.argv[3], 'r' ): IOError: [Errno 13] Permission denied: '/scratch/XXXXXX/XXXXXX/galaxy/tmp/tmpSlG2pC' The permissions on the referenced file: -rw-------+ 1 rlemley rlemley 317 Mar 7 13:42 tmpSlG2pC As the Galaxy daemons run as XXXXXX_galaxy, it does not have permission to read the file. I tried modifying external_chown_script.py so that XXXXXX_galaxy has permission to read that file, but that caused problems elsewhere so I backed out the changes. Any suggestions? Many thanks, -Rob Rob Lemley Systems Administrator - CIRC University of Rochester