readShuffle.pl $input $output **What it does** This tool shuffles sequences in a FASTA file. The output FASTA will have the same composition of nucleotides, in a random order. -------- **Purpose** Allows the user to create a random "in silico" library for statstical analysis. Usage example: analyze your original FASTA library, then shuffle the FASTA file and perform the same analysis on the shuffled file (as a control). -------- **Example** Input FASTA file:: >BILLIE_1_FC00042:2:1:1465:771 TTAGAGACTACTGCTGACATTTTCTGTCTCTGATAT >BILLIE_1_FC00042:2:1:1480:719 CTCGGTGGTAGTTGGCGCACTCCTCTACGATTATAA >BILLIE_1_FC00042:2:1:1617:999 AATTTAGCTATGCGATAACCAGCCTCTTAATGATGG Output sequence (nucleotides are in random order):: >BILLIE_1_FC00042:2:1:1465:771 GACTTAATATTTTTGTTTCGTCTGTAGGCACCATCA >BILLIE_1_FC00042:2:1:1480:719 GTGCATTGTAGTCGCATTGTCCTGTAGGCACCATCA >BILLIE_1_FC00042:2:1:1617:999 GATCATTTTGAAAGCTGATTCTGTAGGCACCATCAA