Hi, I have a 80 core/1TB RAM Dell server. In order improve Galaxy performance I modified the universe_wsgi.ini file so as to improve the Scaling and Load Balancing as follows: # Use a threadpool for the web server instead of creating a thread for each # request. use_threadpool = True # Number of threads in the web server thread pool. threadpool_workers = 200 ################################################################################ [server:web0] use = egg:Paste#http port = 8090 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web1] use = egg:Paste#http port = 8091 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web2] use = egg:Paste#http port = 8092 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web3] use = egg:Paste#http port = 8093 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web4] use = egg:Paste#http port = 8094 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web5] use = egg:Paste#http port = 8095 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web6] use = egg:Paste#http port = 8096 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web7] use = egg:Paste#http port = 8097 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web8] use = egg:Paste#http port = 8098 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web9] use = egg:Paste#http port = 8099 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 [server:web10] use = egg:Paste#http port = 8100 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10 ################################################################################ [server:manager] use = egg:Paste#http port = 8200 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 ################################################################################ [server:handler0] use = egg:Paste#http port = 8300 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler1] use = egg:Paste#http port = 8301 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler2] use = egg:Paste#http port = 8302 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler3] use = egg:Paste#http port = 8303 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler4] use = egg:Paste#http port = 8304 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler5] use = egg:Paste#http port = 8305 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler6] use = egg:Paste#http port = 8306 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler7] use = egg:Paste#http port = 8307 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler8] use = egg:Paste#http port = 8308 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler9] use = egg:Paste#http port = 8309 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler10] use = egg:Paste#http port = 8310 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler11] use = egg:Paste#http port = 8311 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler12] use = egg:Paste#http port = 8312 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler13] use = egg:Paste#http port = 8313 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler14] use = egg:Paste#http port = 8314 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler15] use = egg:Paste#http port = 8315 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler16] use = egg:Paste#http port = 8316 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler17] use = egg:Paste#http port = 8317 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 [server:handler18] use = egg:Paste#http port = 8318 host = 127.0.0.1 use_threadpool = true threadpool_workers = 5 # [server:handler19] # use = egg:Paste#http # port = 8319 # host = 127.0.0.1 # use_threadpool = true # threadpool_workers = 5 # [server:handler20] # use = egg:Paste#http # port = 8320 # host = 127.0.0.1 # use_threadpool = true # threadpool_workers = 5 ################################################################################ job_manager = manager job_handlers = handler0,handler1,,handler2,handler3,handler4,handler5,handler6,handler7,handler8,handler9,handler10,handler11,handler12,handler13,handler14,handler15,handler16,handler17,handler18 ################################################################################ Now the problem I have is that when I start galaxy I cannot get the last [server:handler18] to load correclt I get the following error: galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,236 Enabling 'library_contents' API controller, class: LibraryContentsController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,236 Enabling 'group_users' API controller, class: GroupUsersAPIController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,236 Enabling 'configuration' API controller, class: ConfigurationController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,237 Enabling 'requests' API controller, class: RequestsAPIController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,238 Enabling 'folders' API controller, class: FoldersController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,238 Enabling 'quotas' API controller, class: QuotaAPIController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,239 Enabling 'folder_contents' API controller, class: FolderContentsController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,239 Enabling 'roles' API controller, class: RoleAPIController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,240 Enabling 'request_types' API controller, class: RequestTypeAPIController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,240 Enabling 'users' API controller, class: UserAPIController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,241 Enabling 'samples' API controller, class: SamplesAPIController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,241 Enabling 'forms' API controller, class: FormDefinitionAPIController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,241 Enabling 'permissions' API controller, class: PermissionsController galaxy.web.framework.base DEBUG 2013-08-05 17:08:24,242 Enabling 'workflows' API controller, class: WorkflowsAPIController galaxy.webapps.galaxy.buildapp DEBUG 2013-08-05 17:08:24,242 app.config.api_folders: False galaxy.webapps.galaxy.buildapp DEBUG 2013-08-05 17:08:24,450 Enabling 'httpexceptions' middleware galaxy.webapps.galaxy.buildapp DEBUG 2013-08-05 17:08:24,450 Enabling 'recursive' middleware galaxy.webapps.galaxy.buildapp DEBUG 2013-08-05 17:08:24,700 Enabling 'error' middleware galaxy.webapps.galaxy.buildapp DEBUG 2013-08-05 17:08:24,701 Enabling 'trans logger' middleware galaxy.webapps.galaxy.buildapp DEBUG 2013-08-05 17:08:24,701 Enabling 'x-forwarded-host' middleware galaxy.webapps.galaxy.buildapp DEBUG 2013-08-05 17:08:24,701 Enabling 'Request ID' middleware Starting server in PID 3899. Traceback (most recent call last): File "./scripts/paster.py", line 33, in <module> serve.run() File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/serve.py", line 1049, in run invoke(command, command_name, options, args[1:]) File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/serve.py", line 1055, in invoke exit_code = runner.run(args) File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/serve.py", line 220, in run result = self.command() File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/serve.py", line 670, in command serve() File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/serve.py", line 654, in serve server(app) File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/loadwsgi.py", line 292, in server_wrapper **context.local_conf) File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/loadwsgi.py", line 100, in fix_call reraise(*exc_info) File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/loadwsgi.py", line 37, in reraise exec('raise t, e, tb', dict(t=t, e=e, tb=tb)) File "/usr/local/galaxy/galaxy-dist/lib/galaxy/util/pastescript/loadwsgi.py", line 97, in fix_call val = callable(*args, **kw) File "/usr/local/galaxy/galaxy-dist/eggs/Paste-1.7.5.1-py2.7.egg/paste/httpserver.py", line 1342, in server_runner serve(wsgi_app, **kwargs) TypeError: serve() got an unexpected keyword argument 'job_manager' Removing PID file handler18.pid If I use activate [server:handler20] for example, this one would act up as well. It is always the last one and in all cases the port used is totally incorrect and was never assigned Am I doing something terribly wrong? I tried to use ports not 'assigned' and tried to add web servers and handlers according to my best understanding of the instructions (http://wiki.galaxyproject.org/Admin/Config/Performance/Web%20Application%20S...) Please any suggestions would be most welcome Thanks --Rodolfo