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Hi, I noticed that there is a parameter: Maximum insert size for valid paired-end alignments (-X) for bowtie on Galaxy. The default value on Galaxy is -X 1000. I was wondering if this is the default value we should use ? Or we need to change it to another value ? Thanks a lot. Jianpeng ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).