Hi again Kanwei, I've got a few more BLAST+ wrapper changes, most of this is adding unit tests which just assume BLAST 2.2.24+ is installed (they do not use any databases, just FASTA file vs FASTA file). I also fixed the binary dependency declarations (all five wrappers said they depended on the blastn binary, which was sloppy of me). Note that currently Galaxy doesn't use this information, see Issue 82: http://bitbucket.org/galaxy/galaxy-central/issue/82/fix-the-tag-set-in-the-t... All the changes are on this new branch, http://bitbucket.org/peterjc/galaxy-central/src/blastplus_nov18 Change sets are: http://bitbucket.org/peterjc/galaxy-central/changeset/4f37444d4038 http://bitbucket.org/peterjc/galaxy-central/changeset/cf6c71e06097 http://bitbucket.org/peterjc/galaxy-central/changeset/3b11c4d0a22c http://bitbucket.org/peterjc/galaxy-central/changeset/54be89e916b9 http://bitbucket.org/peterjc/galaxy-central/changeset/94b013214fd3 To run the new tests, use: ./run_functional_tests.sh -id ncbi_blastp_wrapper ./run_functional_tests.sh -id ncbi_tblastn_wrapper Please let me know if there are any problems with the tests on your Galaxy -- for example discrepancies in the output due to the BLAST+ version installed. Regards, Peter