Dear All, When I turn on Nginx upload (as below) I get and error: Not Found The resource cannot be found No route for /data/database/tmp/upload_store The nginx upload settings for universe.ini are: # nginx can also handle file uploads (user-to-Galaxy) via nginx_upload_module. # Configuration for this is complex and explained in detail in the # documentation linked above. The upload store is a temporary directory in # which files uploaded by the upload module will be placed. nginx_upload_store = True # This value overrides the action set on the file upload form, e.g. the web # path where the nginx_upload_module has been configured to intercept upload # requests. nginx_upload_path = /data/database/tmp/upload_store The nginx.conf settings are: location /_upload { upload_store /data/database/tmp/upload_store; upload_pass_form_field ""; upload_set_form_field "__${upload_field_name}__is_composite" "true"; upload_set_form_field "__${upload_field_name}__keys" "name path"; upload_set_form_field "${upload_field_name}_name" "$upload_file_name"; upload_set_form_field "${upload_field_name}_path" "$upload_tmp_path"; upload_pass_args on; upload_pass /_upload_done; } location /_upload_done { set $dst /tool_runner/index; if ($args ~ nginx_redir=([^&]+)) { set $dst $1; } rewrite "" $dst; } } If I comment out the 2 universe.ini settings above then the upload works correctly. I would appreciate any suggestions, Yours Bart.