Dannon, I've set up galaxy at the local linux. After data configuration, I run fastqc to check how it runs. Here I got error message as below -- error An error occurred running this job: Traceback (most recent call last): File "../galaxy-dist/tools/rgenetics/rgFastQC.py", line 158, in assert os.path.isfile(opts.executable),'##rgFastQC.py error - cannot find executable %s' % opts.executable AssertionError: ##r Here is the detail of the error -- Tool: FastQC:Read QC Name: FastQC_FASTQ Groomer on data 6.html Created: May 02, 2013 Filesize: 0 bytes Dbkey: mm9 Format: html Tool Version: Tool Standard Output: stdout Tool Standard Error: stderr Tool Exit Code: 1 Input Parameter Value Short read data from your current history 8: FASTQ Groomer on data 6 Title for the output file - to remind you what the job was for FastQC Contaminant list No dataset What might be the problem? Thank you! Kathryn