Hello everyone, I installed both Galaxy and Apache in a Red Hat linux machine and they work fine individually. If I open http://localhost then a welcome page comes out showing the httpd is working. In galaxy I changed host=0.0.0.0 and "sh run.sh", then the http://localhost<http://localhost/>:8080 will direct me to the Galaxy page. Now I want to put Galaxy under Apache and here is what I did: 1. First I comment the "host=0.0.0.0" line to stop the build in web server in Galaxy and then "sh run.sh" to make Galaxy running. 2. Then I put these lines RewriteEngine On RewriteRule ^/galaxy$ /galaxy/ [R] RewriteRule ^/static/style/(.*) /home/galaxy/galaxy-dist/static/june_2007_style/blue/$1 [L] RewriteRule ^/static/scripts/(.*) /home/galaxy/galaxy-dist/static/scripts/packed/$1 [L] RewriteRule ^/static/(.*) /home/galaxy/galaxy-dist/static/$1 [L] RewriteRule ^/favicon.ico /home/galaxy/galaxy-dist/static/favicon.ico [L] RewriteRule ^/robots.txt /home/galaxy/galaxy-dist/static/robots.txt [L] RewriteRule ^/galaxy(.*) http://localhost:8080$1 [P] into my "httpd.conf" file. Then I restart the httpd service. Also, I made changes to the "universe_wsgi.ini" file as instructed and restart Galaxy: [filter:proxy-prefix] use = egg:PasteDeploy#prefix prefix = /galaxy [app:main] filter-with = proxy-prefix cookie_path = /galaxy After I do these, either http://localhost<http://localhost/>:8080 nor http://localhost<http://localhost/>/galaxy works. I find two threads talked about this problem before: http://user.list.galaxyproject.org/Installing-galaxy-with-Apache-td4654499.h... http://dev.list.galaxyproject.org/Running-Galaxy-behind-apache2-td4624545.ht... but my Red Hat httpd does not has this "/etc/apache2/sites-available/default" directory and only has "/etc/httpd/conf/httpd.conf". Could someone please tell me where should I put those lines in the "httpd.conf" file? I have tries various places and none of them works. Any help would be deeply appreciated. Thanks, Jim