Dear colleagues, I have been struggling with this issue for quite a while now, and I don't seem to find a solution (neither in situ nor in webo). here it is: Because of the confidentiality of our data, I have setup both a Galaxy server and a UCSC Genome Browser locally on my server. Both are run through Apache, using the same .htpasswd authentication method and file (ie: same usernmae/password combo for each). Everything works great except that I do not seem to be able to send/receive data from Galaxy to UCSC and vice versa. When I try to "get data" from UCSC main (my local instance), I get the followin error: "Tool execution generated the following error message: The remote data source application may be off line, please try again later. Error: EOF when reading a line The tool produced the following additional output: Enter username for SG at 127.0.0.1:" where SG is the AuthName in vhost.conf and 127.0.0.1 is UCSC's IP. Whenever I try to to send data to UCSC ("Display at UCSC main" in the history boxes), I get this guy: "Expected 200 http://127.0.0.3/display_application/48916fac0de9a85d/ucsc_bigwig/main/b9ba5...: 401 Authorization Required" Where 127.0.0.3 is galaxy's IP. I have: - hardcoded username:passowrd on UCSC's "send to galaxy" routine but to no avail - hardcode username:passowrd on Galaxy's definiton of UCSC's "main" URL, but to no avail - I have removed UCSC authentication, and everything works fine - I have kept UCSC authentication and let Galaxy "open", doesn't work - I remove authentication to both servers, and it all works cool. Somehow, Galaxy doesn't like to talk to a protected UCSC browser. I'll be happy to post my vhost.conf files if needed. Can anyone help, please? Thanks a lot, Fred -- Federico De Masi, PhD, Assistant Professor The Technical University of Denmark - DTU Center for Biological Sequence Analysis - CBS Kemitorvet 208/002 DK-2800 KGS. LYNGBY, DENMARK Telephone: (+45) 45 25 24 21 Fax: (+45) 45 93 15 85 http://rg.cbs.dtu.dk