Am 03.04.2014 04:34, schrieb Wang, Xiaofei:
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
You mean "SnpEff Download" under Data Manager? But, there is no response when I click on it.
It's a little bit complicated. If you click on the "SnpEff Download" under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool "SnpEff Databases" there you can choose which genome annotation you want to install. To sum it up. 1. click "SnpEff Download" 2. choose one genome from "SnpEff Databases" and install it
Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin > Data > Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why?
Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel? Cheers, Bjoern
When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time?
Thank you so much!
Best,
Xiaofei
________________________________________ From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
* Access data managers - get data, build indexes, etc
* SnpEff Databases<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4> - Read the list of available snpEff databases
* SnpEff Download<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4> - Download a new database
* BWA index<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1> - builder
* BWA Color index<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1> - builder
* Reference Genome<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1> - fetching
* View managed data by manager
* SnpEff Databases<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1> - Read the list of available snpEff databases
* SnpEff Download<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1> - Download a new database
* BWA index<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1> - builder
* BWA Color index<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1> - builder
* Reference Genome<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1> - fetching
* View managed data by Tool Data Table
* snpeff_databases<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases>
* snpeff_genomedb<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb>
* snpeff_regulationdb<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb>
* snpeff_annotations<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations>
* bwa_indexes<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes>
* bwa_indexes_color<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color>
* all_fasta<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta> * * * * ________________________________________ * From: Björn Grüning [bjoern.gruening@gmail.com] * Sent: Wednesday, April 02, 2014 12:55 PM * To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu * Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * * Hi, * * that means that the snpeff annotation is missing. You can install it * with the snpeff datamanager under your Admin panel. Have a look at the * wiki page about data-manager and the snpeff wrapper documentation. * You do not need to edit xml files. If so that is an bug in the wrapper * and we should fix it :) * * Cheers, * Bjoern * * Am 02.04.2014 19:34, schrieb Wang, Xiaofei: * >>> When I used SnpEff in local galaxy instance, I got an error: * >>> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > * > * > * >> Which Java version do you use. Please make sure you are running Java 1.7. * > * > * > * > * > Yes, it is figured, when I changed the java version to 1.7. Thank you so much! * > * > * > * > * > But, I got another error for snpEff. * > * > * > * > * > java.lang.RuntimeException: Property: 'dm5.30.genome' not found * > at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) * > * > * > * > * > * > * > * > * > What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply. * > * > * > * > * > Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? ) * > * > * > * > * > <param name="genomeVersion" type="select" label="Genome"> * > <option value="hg37">Human (hg37)<option> * > <option value="mm37.61">Mouse (mm37.61)<option> * > <param> * > * > * > * > ________________________________________ * > From: Björn Grüning [bjoern.gruening@gmail.com] * > Sent: Wednesday, April 02, 2014 10:35 AM * > To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu * > Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * > * > Hi Xiaofei, * > * >> Dear there, * >> * >> When I used SnpEff in local galaxy instance, I got an error: * >> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > Which Java version do you use. Please make sure you are running Java 1.7. * > * >> Also, when I used the FASTQ Summary Statistics, I got an error like this: * >> Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/... * > * > Can you give us the complete error message. The important part is missing. * > * > Ciao, * > Bjoern * > * >> I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! * >> * >> * >> * >> * >> ___________________________________________________________ * >> Please keep all replies on the list by using "reply all" * >> in your mail client. To manage your subscriptions to this * >> and other Galaxy lists, please use the interface at: * >> http://lists.bx.psu.edu/ * >> * >> To search Galaxy mailing lists use the unified search at: * >> http://galaxyproject.org/search/mailinglists/ * >> * > * > * *