Stephen,,, Here is your solution: In the Tools panel select "Get Data", "Upload File". You can either upload a file, or enter one or more URLs in the "URL/Text" box. Enter "http://galaxy.psu.edu/CPMB/TAF1_ChIP.txt" in the box and click "Execute" I am not sure what happened there but it seems this triggered some database updates that was needed. It worked :).... From: galaxy-dev-bounces@lists.bx.psu.edu [mailto:galaxy-dev-bounces@lists.bx.psu.edu] On Behalf Of Stephen Groenewold Sent: Friday, February 01, 2013 10:41 AM To: galaxy-dev@lists.bx.psu.edu Subject: [galaxy-dev] File Upload doesn't generate any activity We've installed a local instance of Galaxy and are having trouble with the first step of uploading files. The only thing that will currently upload is text pasted into the URL/Text box. Any attempt to upload a file using the Browse File box not only fails, it never even starts. It's as if the Execute button is disabled. I've tried changing the ajax-upload parameter to "false" in tools/data_source/upload.xml (as mentioned in a workaround posted to the list by Daniel Blankenburg in Aug 2012). I've also tried using different browsers (including Internet Explorer) and going the route of Adding Data Libraries as Admin. None of those attempts have worked. Am I missing a configuration setup? Could there be permissions issues on the Galaxy machine? Thanks. Steve