Kip; Are these binary BAM or BigWig type files you are having trouble displaying at UCSC? I don't believe the Paste server has support for the byte-range requests that UCSC needs, and you need to send files through the proxy server. I've done this with nginx so don't have direct Apache experience here, but the 'Sending files using Apache' section here should get it working: http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy Brad
Perhaps this is a distribution issue. For those who have gotten the "Display at UCSC" link to work on a local server or workstation, what linux distribution and version did you use? Has anyone used this successfully with Red Hat Enterprise Linux 6.1?
Best,
-Kip
On Fri, Oct 7, 2011 at 6:28 PM, Bodi, Kip <Kip.Bodi@tufts.edu> wrote:
Hi all,
I have a local galaxy instance, proxied by Apache, and am trying to get the 'display at UCSC' link to work. I've followed all of the instructions and am nearly there, but now I'm getting this response from UCSC when it tries to load the track:
"Byte-range request was ignored by server. Expected Partial Content 206." etc.
I think the error is because the PasteWSGIServer isn't allowing byte-range requests. When I look at the header, "Accept-Ranges: bytes" is not present: [galaxy@galaxy galaxy_dist]$ curl --head localhost HTTP/1.1 200 OK Date: Fri, 07 Oct 2011 22:23:49 GMT Server: PasteWSGIServer/0.5 Python/2.6.6 content-type: text/html; charset=UTF-8 Set-Cookie: galaxysession=c6ca0ddb55be603a4e98b4f13a4db8bfb8635f0aa576697114d40b13a8355efe2cf7a075ef81d769; expires=Thu, 05-Jan-2012 17:23:49 GMT; Max-Age=7776000; Path=/; Version=1 Vary: Accept-Encoding Connection: close
Whereas on another server, "Accept-Ranges: bytes" appears in the header: [galaxy@galaxy galaxy_dist]$ curl --head apollo.med.tufts.edu< http://apollo.med.tufts.edu> HTTP/1.1 403 Forbidden Date: Fri, 07 Oct 2011 22:22:42 GMT Server: Apache/2.2.21 (Fedora) Accept-Ranges: bytes Content-Length: 4609 Connection: close Content-Type: text/html; charset=UTF-8
I'm running RHEL6 with the latest version of galaxy-dist. I've tried manually enabling 'accept-ranges' but haven't had any luck. Any suggestions? Can I enable it somehow in universe_wsgi.ini?
Best,
-Kip