Hello! I am a new user of Galaxy, I believe I am working on the MAIN (although I am not sure). I would like to use the PICRUSt tool on Galaxy through the web. I have created an account, but I did not download Galaxy. I uploaded two .biom files generated in QIIME (see attached). The Greengenes reference database that was used to generate these OTU tables is greengenes/gg_12_10 version. On the web-based PICRUSt tool in Galaxy, I selected the only other option than the current Greengenes database, which is listed as May18 2012. When I try to run normalize_by_copy_number.py I get the following error return for both files: Traceback (most recent call last): File "/net/rcstore02/ifs/rc_labs/galaxy/www/galaxy_hutlab14/galaxy-hutlab14-20150109/tools/picrust_galaxy/scripts/normalize_by_copy_number.py", line 146, in <module> main() File “/net/rcstore02/ifs/rc_labs/galax I am at a loss as to what this error term means. I suspect it is a format issue? But not sure what the issue is or how to fix it. Or that I am using the gg_12_10 version? Cheers Becks Rebecca Prescott, Ph.D University of Hawaii Manoa Department of Microbiology 2538 McCarthy Mall Honolulu, HI 96822