Ok I see that I shouldn't be using FASTQ splitter since the fastq file is already split.
The sequence name is the same for forward and reverse so nothing to key on to force the split. Need a tool that will assume first sequence is forward and second is reverse. Any suggestions? I can't find anything searching messages.
Working with some fastq files that were exported from CLC Genomics Workbench and trying to split them. After running groomer on the files to convert to sanger running splitter and results in 0 split. I have included the first X sequences from the groomer
file. Any thoughts on why it isn't splitting? Is it expecting a particular data pattern for the sequence id? The files originated as qseq on casava 1.7 and were converted to fastq using CLC and the reason the sequence identifier is a little different.
@HWUSI-EAS1775_1_1_6763_945
NGGGCCCCTCCTGAGCCCCAGCCCAGCCCCTCAGGGTGCTTANATCAAGGAGCCTGCCTGTCTGCATGGACCACACGAAAATCTCAAAGGAGTCCTGCGCC
+
#33239988222B@BBBBBBBBB@@@@BBB@22@@@@B@@66#3566666@BBBBB@BB@@@@@B@@B@@B@BB@@@B@@@@@;@@@@@;<<:<<@@@@@<
@HWUSI-EAS1775_1_1_6763_945
CACCTCTCCACGCCCCACCCCGTTCTCCTGGGTGGCGGGGGATGCGAGCGGTGGGATCGTCTCGGCAGGGGGGCAGGACTCCTTTGGGATTTTCGTGTGGT
+
B@B0=?:=0?BB#########################################################################################
@HWUSI-EAS1775_1_1_10085_933
NACCATGTTAGGCTGGTCTCTTAACTCCTGACCTCAGGTGATNTGCTTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGTGCCCAGCC
+
#333366666@BBB@BBBBBB@BBB@BB@BBBB@B@B@@@24#4466666BBB@@BBBBB22B@@@@BB@@@@@@@@@B@@BB@@@@@@@<<<<<@@@@@5
@HWUSI-EAS1775_1_1_10085_933
CCTAATTCATCCACAAGCACCAGAGAATGGGCTGGTGCATGCATGAGTATGCTGGCAGTTTCACTTAATTCAACAAAAAATGGACTTTCACCTTAAAAAAA
+
B::=<9B@?-=7=8+@8@2?91+168@::+5=911B2B:-=:A9:422-,8=42@AB-3BBB3BB=88@@BBBBB?=?==4,+;>BBBB=BB,B3BBBBA#
@HWUSI-EAS1775_1_1_12647_946
NCAGGCAGTGCCCCATAATCCCCTGGGTTTTCTGCATCCCCTNAGTGTCGCCACCGGGTCGCACCTGGCAGGTGCCTTGGCTGCAGCCGCGCCCCACTCCC
+
#211178877@BBBB@BB@B@BBB@B@@BBB@22B@B@B@87#7763666BBBB@@BBBB@BB@B@@@@@:::::82858<<::<@@@@@###########
@HWUSI-EAS1775_1_1_12647_946
CACCCAGCAGGCGCTGCAGCGGGACCTGAAGACCCGGGAGAAGGGGGGGGGGGCGGGGGGGGGGGGGGCCGGGCTGCGGCCCAGGCACCCGCCAGGCGAGA
+
>87-*1:91+=;28+=16:=:5(7:?-B8?:@;=BBBB336=3?#########################################################
@HWUSI-EAS1775_1_1_14406_953
NCTCATATCTGCAAAGAACAGACCGTTCAGTATATACTAACTATGGTGGATGATATGCTGCAGGTAGGTATATTCTTTACTTAACACATTTGGTGACTTCA
+
#2.,.77777B@BBB@@22@@@B@BBBB@B@@@@B@@@@@@B@B@@@@@@@@@@@@B@@@BBBBB@@@8@@@@@@@B22@@@@@@BBB@@@@@@@@B@@@@
@HWUSI-EAS1775_1_1_14406_953
TCTCCCCAAAAATGCTGCTACTGGGAGGGATGCAAAAAAATGAAGTCACCAAATGGGTTAAGTAAAGAATATACCTACCTGCAGCATATCATCCACCATAG
+
A88?1B><?>BB<B=*;6*;=2=+=/:4'18=@@BBBBBB>BBBB6=28@>BB>B57=BBBB<B<B?><;4*1==5>BB>AB3B31+01:B8@B?BBB8@2
@HWUSI-EAS1775_1_1_15514_936
NAAGTACACAGCTACATCCTCAAAGGTTACCAGCTCCTGAAANAACACGTGCTGGCATGCAATTTCAGGGTGAGGAATGTGAATGGCGGCACAAAAAAAAT
+
#/2//87777BBBB@BBB@@B@B@@B@@@@@@@@B@B@@@54#2-,1313@BB@BB@B@BB@BB@@@@@@<<<<<<:<<<@@@@@@@@@@@@@@@222220
@HWUSI-EAS1775_1_1_15514_936
TCTATCCTGGACTCCTGTCTCCCTTCCTCCACCCTGGCCTCCCTTCCTCCCTTCCCAAATGGGCCACTTTCACCCCATCCTCAGTGATTTTTTTTGTGCCG
+
?61-+:*?:;1;1=8);4:-@,@8ABB8BB,?B<2=,;@8BB<;ABBABBB?BBBBBBBA1'-.+%)5;9414:8@93B>+:.73?+?B?<A@@=302BB#
@HWUSI-EAS1775_1_1_16607_936
NGTCTCTCTCCTTTCTGAACAGAGATGAGCCCTAAGATACCTNGAAGATTTTGTTTTTAACCTCACATTAAGGAAAGCACACATCACCTTGAAAGGTTGCA
+
#111/87787@BBB@@@@B@@@@@@?@@@@@@@@@@B@B@66#6655225@@B222222@@B@B@B@@@@@@@B@@@@@@BB@@@B@@@@@B@@@@@@@@:
@HWUSI-EAS1775_1_1_16607_936
TCTGTGCAATGGCACCCTTCTAGGAGGAAGTGTGCAACCTTTCAAGGGGATGTGTGCTTTCCTTAATGGGGGGTTAACAAACACATCTTCCAGGGATTTTA
+
B38?;=:8;5?B?4?3+3++45;>3@1=;>17*1=4BBB?BBB3>BB+3+;@7;1>=ABBBBBB3BB=-31330=BBBB82.B,?BB@=BB?B########