Hello, I and other members of my lab are encountering issues uploading (some) files to the Main public Galaxy server. We routinely upload from our local server to main.g2.bx.psu.edu via ftp using Cyberduck. In my case, on 12/6/12 I uploaded 12 zipped fastq files. Of these, 9 were completely fine. For the remaining 3 files, the transfer through Cyberduck appeared to work fine, and the files appeared (with the correct file size) on the upload screen under "Get data" as usual. However, once the files were uploaded into a Galaxy history, they were empty with a message saying "Problem decompressing gzipped data." An example is entry #45: '1PositiveRFP92112Pool41_ATCACG_L002_R1_001.fastq.gz' in my history called "Tcell_120812." My account uses this email address as the login ID (mmoore@rockefeller.edu). All of these files are pretty large (from 5 to 10 GB once unzipped), but the failure/success did not appear to correlate to the size of the file. I am not exceeding my space quota. In addition, I was able to successfully upload the file listed above (1PositiveRFP92112Pool41_ATCACG_L002_R1_001.fastq.gz) to our local Galaxy installation, so I don't think there is anything wrong with the file itself. However, it has failed to upload to the public galaxy server multiple times. The experience of my lab mates is similar; some of their files are uploading correctly, and others are having the same problem I described above. We can't seem to find anything common among the files that are failing to upload. For instance, they span different sequencing runs and were deposited on our local server at different times Has anyone else been encountering similar issues ? Please let me know if I should direct this question elsewhere or if I can provide any further information. Thanks very much for your time. Michael Moore Rockefeller University