Hi Moritz,

Pls see below

On Jul 26, 2013, at 6:37 AM, Moritz Juchler <juchler@stud.uni-heidelberg.de> wrote:

Hey,

1 I installed Galaxy locally.

2 I also have BWA installed and then used the 

3 shed repository to get the bwa wrapper.

4 I have the reference genome:

-rw-r--r-- 1 trr users      10509 2013-07-25 11:31 human_g1k_v37.dict
-rw-r--r-- 1 trr users 3153506519 2013-07-25 11:28 human_g1k_v37.fasta
-rw-r--r-- 1 trr users       6597 2013-07-25 19:41 human_g1k_v37.fasta.amb
-rw-r--r-- 1 trr users       6844 2013-07-25 19:41 human_g1k_v37.fasta.ann
-rw-r--r-- 1 trr users 3101804844 2013-07-25 19:41 human_g1k_v37.fasta.bwt
-rw-r--r-- 1 trr users       2746 2013-07-25 11:32 human_g1k_v37.fasta.fai
-rw-r--r-- 1 trr users  775451186 2013-07-25 19:41 human_g1k_v37.fasta.pac
-rw-r--r-- 1 trr users 1550902424 2013-07-25 20:02 human_g1k_v37.fasta.sa

 

5 and I changed the bwa_index and bw_index_color.loc to the path

/genedata/human_genome_GRCh37/hg19.fa

This is the problem - the path must point to the full name of the fasta file. In your case "human_g1kv_v37.fasta".

Using the name without the fasta as the base name in other fields as the unique "dbkey" is a good practice.

Hopefully this helps,

Jen

Galaxy team

6 This is my $PATH

trr@portalmoritz:~> echo $PATH
/home/trr/bin:/usr/local/bin:/usr/bin:/bin:/usr/bin/X11:/usr/X11R6/bin:/usr/games:/home/trr/bpipe-0.9.8/bin:/home/trr/bwa-0.7.5a:/home/trr/samtools-0.1.19

Is there anything I am missing? I would be glad about some help.

Best

Moritz

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