I too had trouble with BAM files as https://github.com/galaxyproject/galaxy/blob/dev/lib/galaxy/datatypes/binary... Line 201 class Bam( Binary ): ... def _get_samtools_version( self ): ... samtools_exec = which('samtools') Assumes samtools is installed on the server and was not fining one. I had to install samtools outside of galaxy to get past that. Christian University of Manchester ________________________________ From: galaxy-dev [galaxy-dev-bounces@lists.galaxyproject.org] on behalf of Scott Szakonyi [Scott.B.Szakonyi.1@nd.edu] Sent: Friday, February 26, 2016 5:49 PM To: Galaxy Dev Mailing List Subject: [galaxy-dev] Difficulty uploading BAM files Hi, I'm having trouble uploading BAM files in our development environment. Other files types are uploading without issue. I'm testing with a small BAM file I downloaded, and I'm able to successfully upload it on our production server and usegalaxy.org<http://usegalaxy.org>. When I try to upload in the development environment, I get the following error: Traceback (most recent call last): File "/vectorbase/web/Galaxy/galaxy/tools/data_source/upload.py", line 431, in <module> __main__() File "/vectorbase/web/Galaxy/galaxy/tools/data_source/upload.py", line 420, in __main__ add_file( dataset, registry, json_file, output_path ) File "/vectorbase/web/Galaxy/galaxy/tools/data_source/upload.py", line 347, in add_file if link_data_only == 'copy_files' and datatype.dataset_content_needs_grooming( output_path ): File "/vectorbase/web/Galaxy/galaxy/lib/galaxy/datatypes/binary.py", line 218, in dataset_content_needs_grooming version = self._get_samtools_version() File "/vectorbase/web/Galaxy/galaxy/lib/galaxy/datatypes/binary.py", line 179, in _get_samtools_version raise Exception(message) Exception: Attempting to use functionality requiring samtools, but it cannot be located on Galaxy's PATH. I've checked my installed tools, and all the same Samtools packages are successfully installed on the development environment as in production. I don't see any missing dependencies or anything like that. At this point I'm stumped. If anyone can offer some guidance on how to resolve this, I'd be most appreciative. Best regards, -- Scott B. Szakonyi Research Programmer Center for Research Computing 107 Information Technology Center Notre Dame, IN 46556 http://crc.nd.edu<http://crc.nd.edu/>