Hi I installed Galaxy on Mac OS 10.6.4, everything was working fine until I tried to use Apache server. I followed the directions as per http://bitbucket.org/galaxy/galaxy-central/wiki/Config/ApacheProxy. Added the following to my httpd.cof RewriteEngine on <Location "/"> # Define the authentication method AuthType Basic AuthName Galaxy AuthUserFile /home/nate/htpasswd Require valid-user # Define Galaxy as a valid Proxy <Proxy http://localhost:8080> Order deny,allow Allow from all </Proxy> # Take the $REMOTE_USER environment variable and set it as a header in the proxy request. RewriteCond %{IS_SUBREQ} ^false$ RewriteCond %{LA-U:REMOTE_USER} (.+) RewriteRule . - [E=RU:%1] RequestHeader set REMOTE_USER %{RU}e </Location> RewriteRule ^/static/style/(.*) /home/nate/galaxy_dist/static/june_2007_style/blue/$1 [L] RewriteRule ^/static/(.*) /home/nate/galaxy_dist/static/$1 [L] RewriteRule ^/favicon.ico /home/nate/galaxy_dist/static/favicon.ico [L] RewriteRule ^/robots.txt /home/nate/galaxy_dist/static/robots.txt [L] RewriteRule ^(.*) http://localhost:8080$1 [P] </Location> I am getting a "<Proxy not allowed here" error where I start apache. Any help is appreciated. -Sohail