Hi Pat, Briefly, to get galaxy uploads working, you need to do the following: 1. Install an FTP server. The FTP server can be on the same/different host than galaxy but must be able to access the "ftp_upload_dir" 2. Configure the FTP server to authenticate users. This needs to be the same user ID as the galaxy user ID. For example: user id -> iyad.kandalaft@agr.gc.ca 3. Configure the FTP server to create a folder in "ftp_upload_dir" that has the same name as the user that logged into the FTP server. For example: user id -> iyad.kandalaft@agr.gc.ca directory is -> galaxy_dist/database/files/iyad.kandalaft@agr.gc.ca/ The link you posted takes briefly describes how to configure ProFTPd, which is an FTP server, to work with Galaxy. Unfortunately, the page is somewhat random as it describes steps for some operating systems in detail but leaves out the details for other operating systems. On a redhat/centos operating systems, you install proftpd using: sudo yum install proftpd On Debian/Ubuntu: sudo apt-get install proftpd The configuration file that the tutorial refers to is /etc/proftpd.conf I hope this helps get you started. Regards, Iyad Kandalaft Bioinformatics Programmer Microbial Biodiversity Bioinformatics Science & Technology Branch Agriculture & Agri-Food Canada Iyad.Kandalaft@agr.gc.ca | (613) 759-1228 ________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Pat-74100 [leonardsquall15@hotmail.com] Sent: June 29, 2014 10:29 AM To: galaxy-dev@lists.bx.psu.edu Subject: [galaxy-dev] Set a FTP upload in Local Galaxy instance Dear Galaxy developers I'm trying to allow users to upload file via FTP. I've been to the tutorial website but as a beginner, I understand nothing... https://wiki.galaxyproject.org/Admin/Config/UploadviaFTP?action=show&redirect=Admin%2FConfig%2FUpload+via+FTP I've configured the universe_wsgi.ini file with ftp_upload_dir = galaxy_dist/database/files/ ftp_upload_site = any adress. I use Filezilla to for FTP connecting but it doesn't works and it asks me a Port ... Can someone provide to me a simply tutorial to enable ftp upload ? Thanks Pat