Hello,

I am trying to build Bowtie2 index for hg19.

I a using Docker image of Galaxy.

Computer I am using have 12 cores and 64 GB of RAM.

Error I am getting is:

Fatal error: Exit code 1 ()

Building a SMALL index

Could not allocate space for a joined string of 2897310462 elements.

Switching to a packed string representation.

Building a SMALL index

Could not find approrpiate bmax/dcv settings for building this index.

Already tried a packed string representation.

Please try indexing this reference on a computer with more memory.

Error: Encountered internal Bowtie 2 exception (#1)

Command: bowtie2-build --wrapper basic-0 /export/galaxy-central/database/job_working_directory/000/113/dataset_80_files/hg19 fa.fa hg19 fa

Deleting "hg19 fa.3.bt2" file written during aborted indexing attempt.

Deleting "hg19 fa.4.bt2" file written during aborted indexing attempt.

Deleting "hg19 fa.1.bt2" file written during aborted indexing attempt.

Deleting "hg19 fa.2.bt2" file written during aborted indexing attempt.

Deleting "hg19 fa.3.bt2" file written during aborted indexing attempt.

Deleting "hg19 fa.4.bt2" file written during aborted indexing attempt.

Deleting "hg19 fa.1.bt2" file written during aborted indexing attempt.

Deleting "hg19 fa.2.bt2" file written during aborted indexing attempt.

Error building index.

Please help!

Thank you,

Deepak