Hi Peter,

Here is the full log:


This is MIRA V3.4.0 (production version).

Please cite: Chevreux, B., Wetter, T. and Suhai, S. (1999), Genome Sequence
Assembly Using Trace Signals and Additional Sequence Information.
Computer Science and Biology: Proceedings of the German Conference on
Bioinformatics (GCB) 99, pp. 45-56.

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	http://www.chevreux.org/mira_mailinglists.html

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	mira_talk@freelists.org

To report bugs or ask for features, please use the new ticketing system at:
	http://sourceforge.net/apps/trac/mira-assembler/
This ensures that requests don't get lost.


Compiled by: bach
Sun Aug 21 17:50:30 CEST 2011
On: Linux arcadia 2.6.38-11-generic #48-Ubuntu SMP Fri Jul 29 19:02:55 UTC 2011 x86_64 x86_64 x86_64 GNU/Linux
Compiled in boundtracking mode.
Compiled in bugtracking mode.
Compiled with ENABLE64 activated.
Runtime settings (sorry, for debug):
	Size of size_t  : 8
	Size of uint32  : 4
	Size of uint32_t: 4
	Size of uint64  : 8
	Size of uint64_t: 8
Current system: Linux whsiao-ubuntu 2.6.32-40-generic #87-Ubuntu SMP Tue Mar 6 00:56:56 UTC 2012 x86_64 GNU/Linux



Parsing parameters: --job=denovo,genome,accurate SANGER_SETTINGS -LR:lsd=1:mxti=0:ft=fastq -FN:fqi=/media/partition2_/galaxydb_data/000/dataset_290.dat COMMON_SETTINGS -OUT:orf=1:orc=1:ora=1:orw=1:orm=0:org=0:ors=0 -OUT:rrot=1:rtd=1




Parameters parsed without error, perfect.

-CL:pec and -CO:emeas1clpec are set, setting -CO:emea values to 1.
------------------------------------------------------------------------------
Parameter settings seen for:
Sanger data (also common parameters)

Used parameter settings:
  General (-GE):
	Project name in (proin)                     : mira
	Project name out (proout)                   : mira
	Number of threads (not)                     : 2
	Automatic memory management (amm)           : yes
	    Keep percent memory free (kpmf)         : 15
	    Max. process size (mps)                 : 0
	EST SNP pipeline step (esps)                : 0
	Use template information (uti)              : yes
	    Template insert size minimum (tismin)   : -1
	    Template insert size maximum (tismax)   : -1
	    Template partner build direction (tpbd) : -1
	Colour reads by hash frequency (crhf)       : yes

  Load reads options (-LR):
	Load sequence data (lsd)                    : yes
	    File type (ft)                          : fastq
	    External quality (eq)                   : from SCF (scf)
	        Ext. qual. override (eqo)           : no
	        Discard reads on e.q. error (droeqe): no
	    Solexa scores in qual file (ssiqf)      : no
	    FASTQ qual offset (fqqo)                : 0

	Wants quality file (wqf)                    : yes

	Read naming scheme (rns)                    :  [san] Sanger Institute (sanger)

	Merge with XML trace info (mxti)            : no

	Filecheck only (fo)                         : no

  Assembly options (-AS):
	Number of passes (nop)                      : 4
	    Skim each pass (sep)                    : yes
	Maximum number of RMB break loops (rbl)     : 2
	Maximum contigs per pass (mcpp)             : 0

	Minimum read length (mrl)                   : 80
	Minimum reads per contig (mrpc)             : 2
	Base default quality (bdq)                  : 10
	Enforce presence of qualities (epoq)        : yes

	Automatic repeat detection (ard)            : yes
	    Coverage threshold (ardct)              : 2
	    Minimum length (ardml)                  : 400
	    Grace length (ardgl)                    : 40
	    Use uniform read distribution (urd)     : no
	      Start in pass (urdsip)                : 3
	      Cutoff multiplier (urdcm)             : 1.5
	Keep long repeats separated (klrs)          : no

	Spoiler detection (sd)                      : yes
	    Last pass only (sdlpo)                  : yes

	Use genomic pathfinder (ugpf)               : yes

	Use emergency search stop (uess)            : yes
	    ESS partner depth (esspd)               : 500
	Use emergency blacklist (uebl)              : yes
	Use max. contig build time (umcbt)          : no
	    Build time in seconds (bts)             : 10000

  Strain and backbone options (-SB):
	Load straindata (lsd)                       : no
	Assign default strain (ads)                 : no
	    Default strain name (dsn)               : StrainX
	Load backbone (lb)                          : no
	    Start backbone usage in pass (sbuip)    : 3
	    Backbone file type (bft)                : fasta
	    Backbone base quality (bbq)             : 30
	    Backbone strain name (bsn)              : ReferenceStrain
	        Force for all (bsnffa)              : no
	    Backbone rail from strain (brfs)        : 
	    Backbone rail length (brl)              : 0
	    Backbone rail overlap (bro)             : 0
	    Also build new contigs (abnc)           : yes

  Dataprocessing options (-DP):
	Use read extensions (ure)                   : yes
	    Read extension window length (rewl)     : 30
	    Read extension w. maxerrors (rewme)     : 2
	    First extension in pass (feip)          : 0
	    Last extension in pass (leip)           : 0

  Clipping options (-CL):
	Merge with SSAHA2/SMALT vector screen (msvs): no
	    Gap size (msvsgs)                       : 10
	    Max front gap (msvsmfg)                 : 60
	    Max end gap (msvsmeg)                   : 120
	    Strict front clip (msvssfc)             : 0
	    Strict end clip (msvssec)               : 0
	Possible vector leftover clip (pvlc)        : yes
	    maximum len allowed (pvcmla)            : 18
	Min qual. threshold for entire read (mqtfer): 0
	    Number of bases (mqtfernob)             : 0
	Quality clip (qc)                           : no
	    Minimum quality (qcmq)                  : 20
	    Window length (qcwl)                    : 30
	Bad stretch quality clip (bsqc)             : yes
	    Minimum quality (bsqcmq)                : 20
	    Window length (bsqcwl)                  : 30
	Masked bases clip (mbc)                     : yes
	    Gap size (mbcgs)                        : 20
	    Max front gap (mbcmfg)                  : 40
	    Max end gap (mbcmeg)                    : 60
	Lower case clip (lcc)                       : no
	Clip poly A/T at ends (cpat)                : no
	    Keep poly-a signal (cpkps)              : no
	    Minimum signal length (cpmsl)           : 12
	    Max errors allowed (cpmea)              : 1
	    Max gap from ends (cpmgfe)              : 9
	Clip 3 prime polybase (c3pp)                : no
	    Minimum signal length (c3ppmsl)         : 12
	    Max errors allowed (c3ppmea)            : 2
	    Max gap from ends (c3ppmgfe)            : 9
	Clip known adaptors right (ckar)            : no
	Ensure minimum left clip (emlc)             : yes
	    Minimum left clip req. (mlcr)           : 25
	    Set minimum left clip to (smlc)         : 30
	Ensure minimum right clip (emrc)            : no
	    Minimum right clip req. (mrcr)          : 10
	    Set minimum right clip to (smrc)        : 20

	Apply SKIM chimera detection clip (ascdc)   : yes
	Apply SKIM junk detection clip (asjdc)      : no

	Propose end clips (pec)                     : yes
	    Bases per hash (pecbph)                 : 17
	    Handle Solexa GGCxG problem (pechsgp)   : yes

	Clip bad solexa ends (cbse)                 : yes

  Parameters for SKIM algorithm (-SK):
	Number of threads (not)                     : 2

	Also compute reverse complements (acrc)     : yes
	Bases per hash (bph)                        : 21
	Hash save stepping (hss)                    : 1
	Percent required (pr)                       : 65

	Max hits per read (mhpr)                    : 2000
	Max megahub ratio (mmhr)                    : 0

	SW check on backbones (swcob)               : no

	Freq. est. min normal (fenn)                : 0.4
	Freq. est. max normal (fexn)                : 1.6
	Freq. est. repeat (fer)                     : 1.9
	Freq. est. heavy repeat (fehr)              : 8
	Freq. est. crazy (fecr)                     : 20
	Mask nasty repeats (mnr)                    : yes
	    Nasty repeat ratio (nrr)                : 100
	Repeat level in info file (rliif)           : 6

	Max hashes in memory (mhim)                 : 15000000
	MemCap: hit reduction (mchr)                : 2048

  Pathfinder options (-PF):
	Use quick rule (uqr)                        : yes
	    Quick rule min len 1 (qrml1)            : 200
	    Quick rule min sim 1 (qrms1)            : 90
	    Quick rule min len 2 (qrml2)            : 100
	    Quick rule min sim 2 (qrms2)            : 95
	Backbone quick overlap min len (bqoml)      : 150
	Max. start cache fill time (mscft)          : 5

  Align parameters for Smith-Waterman align (-AL):
	Bandwidth in percent (bip)             : 20
	Bandwidth max (bmax)                   : 130
	Bandwidth min (bmin)                   : 25
	Minimum score (ms)                     : 30
	Minimum overlap (mo)                   : 17
	Minimum relative score in % (mrs)      : 65
	Solexa_hack_max_errors (shme)          : 0
	Extra gap penalty (egp)                : no
	    extra gap penalty level (egpl)     :  [san] low
	    Max. egp in percent (megpp)        : 100

  Contig parameters (-CO):
	Name prefix (np)                                         : mira
	Reject on drop in relative alignment score in % (rodirs) : 25
	Mark repeats (mr)                                        : yes
	    Only in result (mroir)                               : no
	    Assume SNP instead of repeats (asir)                 : no
	    Minimum reads per group needed for tagging (mrpg)    : 2
	    Minimum neighbour quality needed for tagging (mnq)   : 20
	    Minimum Group Quality needed for RMB Tagging (mgqrt) : 30
	    End-read Marking Exclusion Area in bases (emea)      : 1
	        Set to 1 on clipping PEC (emeas1clpec)           : yes
	    Also mark gap bases (amgb)                           : yes
	        Also mark gap bases - even multicolumn (amgbemc) : yes
	        Also mark gap bases - need both strands (amgbnbs): yes
	Force non-IUPAC consensus per sequencing type (fnicpst)  : no
	Merge short reads (msr)                                  : no
	    Keep ends unmerged (msrkeu)                          : -1
	Gap override ratio (gor)                                 : 66

  Edit options (-ED):
	Automatic contig editing (ace)              : no
     Sanger only:
	Strict editing mode (sem)                   : no
	Confirmation threshold in percent (ct)      : 50

  Misc (-MI):
	Stop on NFS (sonfs)                         : yes
	Extended log (el)                           : no
	Large contig size (lcs)                     : 500
	Large contig size for stats(lcs4s)          : 5000
	Stop on max read name length (somrnl)       : 40

  Directories (-DI):
	Working directory                 : 
	When loading EXP files            : 
	When loading SCF files            : 
	Top directory for writing files   : mira_assembly
	For writing result files          : mira_assembly/mira_d_results
	For writing result info files     : mira_assembly/mira_d_info
	For writing tmp files             : mira_assembly/mira_d_tmp
	Tmp redirected to (trt)           : 
	For writing checkpoint files      : mira_assembly/mira_d_chkpt

  File names (-FN):
	When loading sequences from FASTA            : mira_in.sanger.fasta
	When loading qualities from FASTA quality    : mira_in.sanger.fasta.qual
	When loading sequences from FASTQ            : /media/partition2_/galaxydb_data/000/dataset_290.dat
	When loading project from CAF                : mira_in.sanger.caf
	When loading project from MAF (disabled)     : mira_in.sanger.maf
	When loading EXP fofn                        : mira_in.sanger.fofn
	When loading project from PHD                : mira_in.phd.1
	When loading strain data                     : mira_straindata_in.txt
	When loading XML trace info files            : mira_traceinfo_in.sanger.xml
	When loading SSAHA2 vector screen results    : mira_ssaha2vectorscreen_in.txt
	When loading SMALT vector screen results     : mira_smaltvectorscreen_in.txt

	When loading backbone from MAF               : mira_backbone_in.maf
	When loading backbone from CAF               : mira_backbone_in.caf
	When loading backbone from GenBank           : mira_backbone_in.gbf
	When loading backbone from GFF3              : mira_backbone_in.gff3
	When loading backbone from FASTA             : mira_backbone_in.fasta


  Output files (-OUTPUT/-OUT):
	Save simple singlets in project (sssip)      : no
	Save tagged singlets in project (stsip)      : yes

	Remove rollover tmps (rrot)                  : yes
	Remove tmp directory (rtd)                   : yes

    Result files:
	Saved as CAF                       (orc)     : yes
	Saved as MAF                       (orm)     : no
	Saved as FASTA                     (orf)     : yes
	Saved as GAP4 (directed assembly)  (org)     : no
	Saved as phrap ACE                 (ora)     : yes
	Saved as GFF3                     (org3)     : no
	Saved as HTML                      (orh)     : no
	Saved as Transposed Contig Summary (ors)     : no
	Saved as simple text format        (ort)     : no
	Saved as wiggle                    (orw)     : yes

    Temporary result files:
	Saved as CAF                       (otc)     : yes
	Saved as MAF                       (otm)     : no
	Saved as FASTA                     (otf)     : no
	Saved as GAP4 (directed assembly)  (otg)     : no
	Saved as phrap ACE                 (ota)     : no
	Saved as HTML                      (oth)     : no
	Saved as Transposed Contig Summary (ots)     : no
	Saved as simple text format        (ott)     : no

    Extended temporary result files:
	Saved as CAF                      (oetc)     : no
	Saved as FASTA                    (oetf)     : no
	Saved as GAP4 (directed assembly) (oetg)     : no
	Saved as phrap ACE                (oeta)     : no
	Saved as HTML                     (oeth)     : no
	Save also singlets               (oetas)     : no

    Alignment output customisation:
	TEXT characters per line (tcpl)              : 60
	HTML characters per line (hcpl)              : 60
	TEXT end gap fill character (tegfc)          :  
	HTML end gap fill character (hegfc)          :  

    File / directory output names:
	CAF             : mira_out.caf
	MAF             : mira_out.maf
	FASTA           : mira_out.unpadded.fasta
	FASTA quality   : mira_out.unpadded.fasta.qual
	FASTA (padded)  : mira_out.padded.fasta
	FASTA qual.(pad): mira_out.padded.fasta.qual
	GAP4 (directory): mira_out.gap4da
	ACE             : mira_out.ace
	HTML            : mira_out.html
	Simple text     : mira_out.txt
	TCS overview    : mira_out.tcs
	Wiggle          : mira_out.wig
------------------------------------------------------------------------------
Deleting old directory mira_assembly ... done.
Creating directory mira_assembly ... done.
Creating directory mira_assembly/mira_d_tmp ... done.
Creating directory mira_assembly/mira_d_results ... done.
Creating directory mira_assembly/mira_d_info ... done.
Creating directory mira_assembly/mira_d_chkpt ... done.

Tmp directory is not on a NFS mount, good.

Localtime: Thu Apr 19 10:53:58 2012

Loading data (Sanger) from FASTQ files,
Localtime: Thu Apr 19 10:53:58 2012
Counting sequences in FASTQ file: found 1 sequences.
Localtime: Thu Apr 19 10:53:58 2012
Localtime: Thu Apr 19 10:53:58 2012

Checking SCF files (loading qualities only if needed):
 [0%] ....|.... [10%] ....|.... [20%] ....|.... [30%] ....|.... [40%] ....|.... [50%] ....|.... [60%] ....|.... [70%] ....|.... [80%] ....|.... [90%] ....|.... [100%] 
Done.
0 SCF files loaded ok.


Sanger will load 1 reads.
Longest Sanger: 36
Longest 454: 0
Longest IonTor: 0
Longest PacBio: 0
Longest Solexa: 0
Longest Solid: 0
Longest overall: 36
Total reads to load: 1
Reserving space for reads
Reserved space for 11 reads.
Loading data (Sanger) from FASTQ files,
Localtime: Thu Apr 19 10:53:58 2012
Counting sequences in FASTQ file: found 1 sequences.
Localtime: Thu Apr 19 10:53:58 2012
Using calculated FASTQ quality offset: 104
Localtime: Thu Apr 19 10:53:58 2012
Loading data from FASTQ file:
 [0%] ....|.... [10%] ....|.... [20%] ....|.... [30%] ....|.... [40%] ....|.... [50%] ....|.... [60%] ....|.... [70%] ....|.... [80%] ....|.... [90%] ....|.... [100%] 

Done.
Loaded 1 reads, Localtime: Thu Apr 19 10:53:58 2012
Localtime: Thu Apr 19 10:53:58 2012

Checking SCF files (loading qualities only if needed):
 [0%] ....|.... [10%] ....|.... [20%] ....|.... [30%] ....|.... [40%] ....|.... [50%] ....|.... [60%] ....|.... [70%] ....|.... [80%] ....|.... [90%] ....|.... [100%] 
Done.
0 SCF files loaded ok.
1 SCF files were not found (see 'mira_assembly/mira_d_tmp/mira_info_scfreadfail.0' for a list of names).


Loaded 1 Sanger reads.
Total reads loaded: 1
Localtime: Thu Apr 19 10:53:58 2012

Checking SCF files (loading qualities only if needed):
 [0%] ....|.... [10%] ....|.... [20%] ....|.... [30%] ....|.... [40%] ....|.... [50%] ....|.... [60%] ....|.... [70%] ....|.... [80%] ....|.... [90%] ....|.... [100%] 
Done.
0 SCF files loaded ok.
1 SCF files were not found (see 'mira_assembly/mira_d_tmp/mira_info_scfreadfail.0' for a list of names).


Checking reads for trace data:
 [0%] ....|.... [10%] ....|.... [20%] ....|.... [30%] ....|.... [40%] ....|.... [50%] ....|.... [60%] ....|.... [70%] ....|.... [80%] ....|.... [90%] ....|.... [100%] 
No SCF data present in any read, automatic contig editing for Sanger data is now switched off.
1 reads with valid data for assembly.
Localtime: Thu Apr 19 10:53:58 2012

Generated 1 unique template ids for 1 valid reads.
No useful template information found, template routines will not be used.
Localtime: Thu Apr 19 10:53:58 2012

Generated 0 unique strain ids for 1 reads.
Strain "default" has 1 reads.
Have read pool with 1 reads.

===========================================================================
Pool statistics:
Backbones: 0	Backbone rails: 0

		Sanger	454	IonTor	PacBio	Solexa	SOLiD
		----------------------------------------
Total reads	1	0	0	0	0	0
Reads wo qual	0	0	0	0	0	0
Used reads	0	0	0	0	0	0
Avg tot rlen	36	0	0	0	0	0
Avg rlen used	0	0	0	0	0	0

With strain	0	0	0	0	0	0
W/o clips	0	0	0	0	0	0

Sanger	total bases:36	used bases in used reads: 0
454	total bases:0	used bases in used reads: 0
IonTor	total bases:0	used bases in used reads: 0
PacBio	total bases:0	used bases in used reads: 0
Solexa	total bases:0	used bases in used reads: 0
Solid	total bases:0	used bases in used reads: 0
===========================================================================



========================== Memory self assessment ==============================
Running in 64 bit mode.

Dump from /proc/meminfo
--------------------------------------------------------------------------------
MemTotal:       16209628 kB
MemFree:         6527928 kB
Buffers:          324332 kB
Cached:          6344208 kB
SwapCached:          424 kB
Active:          5900208 kB
Inactive:        2224260 kB
Active(anon):    1308772 kB
Inactive(anon):   179636 kB
Active(file):    4591436 kB
Inactive(file):  2044624 kB
Unevictable:          56 kB
Mlocked:              56 kB
SwapTotal:      39535608 kB
SwapFree:       39531904 kB
Dirty:              4292 kB
Writeback:             0 kB
AnonPages:       1455560 kB
Mapped:           187664 kB
Shmem:             32480 kB
Slab:             326072 kB
SReclaimable:     296460 kB
SUnreclaim:        29612 kB
KernelStack:        4000 kB
PageTables:        31452 kB
NFS_Unstable:          0 kB
Bounce:                0 kB
WritebackTmp:          0 kB
CommitLimit:    47640420 kB
Committed_AS:    3610840 kB
VmallocTotal:   34359738367 kB
VmallocUsed:      354268 kB
VmallocChunk:   34359380860 kB
HardwareCorrupted:     0 kB
HugePages_Total:       0
HugePages_Free:        0
HugePages_Rsvd:        0
HugePages_Surp:        0
Hugepagesize:       2048 kB
DirectMap4k:     2587200 kB
DirectMap2M:    13926400 kB
DirectMap1G:           0 kB
--------------------------------------------------------------------------------

Dump from /proc/self/status
--------------------------------------------------------------------------------
Name:	mira
State:	R (running)
Tgid:	20591
Pid:	20591
PPid:	20590
TracerPid:	0
Uid:	0	0	0	0
Gid:	0	0	0	0
FDSize:	64
Groups:	0 
VmPeak:	   11920 kB
VmSize:	   11916 kB
VmLck:	       0 kB
VmHWM:	    7192 kB
VmRSS:	    7192 kB
VmData:	    6556 kB
VmStk:	      88 kB
VmExe:	    5236 kB
VmLib:	       0 kB
VmPTE:	      40 kB
Threads:	1
SigQ:	1/16382
SigPnd:	0000000000000000
ShdPnd:	0000000000000000
SigBlk:	0000000000000000
SigIgn:	0000000001001000
SigCgt:	0000000180000000
CapInh:	0000000000000000
CapPrm:	ffffffffffffffff
CapEff:	ffffffffffffffff
CapBnd:	ffffffffffffffff
Cpus_allowed:	3f
Cpus_allowed_list:	0-5
Mems_allowed:	00000000,00000001
Mems_allowed_list:	0
voluntary_ctxt_switches:	10
nonvoluntary_ctxt_switches:	4
--------------------------------------------------------------------------------

Information on current assembly object:

AS_readpool: 1 reads.
AS_contigs: 0 contigs.
AS_bbcontigs: 0 contigs.
Mem used for reads: 3136 (3 KiB)

Memory used in assembly structures:
                                           Eff. Size   Free cap. LostByAlign
     AS_writtenskimhitsperid:          0        24 B         0 B         0 B
               AS_skim_edges:          0        24 B         0 B         0 B
                 AS_adsfacts:          0        24 B         0 B         0 B
          AS_confirmed_edges:          0        24 B         0 B         0 B
   AS_permanent_overlap_bans:          1        24 B         0 B         0 B
              AS_readhitmiss:          0        24 B         0 B         0 B
            AS_readhmcovered:          0        24 B         0 B         0 B
                AS_count_rhm:          0        24 B         0 B         0 B
                 AS_clipleft:          0        24 B         0 B         0 B
                AS_clipright:          0        24 B         0 B         0 B
                 AS_used_ids:          0        24 B         0 B         0 B
              AS_multicopies:          0        24 B         0 B         0 B
            AS_hasmcoverlaps:          0        24 B         0 B         0 B
       AS_maxcoveragereached:          0        24 B         0 B         0 B
       AS_coverageperseqtype:          0        24 B         0 B         0 B
           AS_istroublemaker:          0        24 B         0 B         0 B
                 AS_isdebris:          0        24 B         0 B         0 B
          AS_needalloverlaps:          0        40 B         0 B         0 B
    AS_readsforrepeatresolve:          0        40 B         0 B         0 B
                AS_allrmbsok:          0        24 B         0 B         0 B
        AS_probablermbsnotok:          0        24 B         0 B         0 B
            AS_weakrmbsnotok:          0        24 B         0 B         0 B
          AS_readmaytakeskim:          0        40 B         0 B         0 B
               AS_skimstaken:          0        40 B         0 B         0 B
          AS_numskimoverlaps:          0        24 B         0 B         0 B
       AS_numleftextendskims:          0        24 B         0 B         0 B
         AS_rightextendskims:          0        24 B         0 B         0 B
      AS_skimleftextendratio:          0        24 B         0 B         0 B
     AS_skimrightextendratio:          0        24 B         0 B         0 B
             AS_usedtmpfiles:          3       112 B         0 B         0 B
Total: 4008 (4 KiB)

================================================================================
Dynamic allocs: 0
Align allocs: 0

Fatal error (may be due to problems of the input data or parameters):

"No read can be used for assembly."

->Thrown: void Assembly::dumpSomeStatistics()
->Caught: main

Aborting process, probably due to error in the input data or parametrisation.
Please check the output log for more information.
For help, please write a mail to the mira talk mailing list.

Subscribing / unsubscribing to mira talk, see: http://www.freelists.org/list/mira_talk

CWD: /usr/local/projects/galaxy-dist/database/job_working_directory/000/253
Thank you for noticing that this is *NOT* a crash, but a
controlled program stop.


MIRA took 0.00 minutes
Return error code 1 from command:
mira --job=denovo,genome,accurate SANGER_SETTINGS -LR:lsd=1:mxti=0:ft=fastq -FN:fqi=/media/partition2_/galaxydb_data/000/dataset_290.dat COMMON_SETTINGS -OUT:orf=1:orc=1:ora=1:orw=1:orm=0:org=0:ors=0 -OUT:rrot=1:rtd=1
Thanks,
Tyler
On Thu, Apr 19, 2012 at 10:48 AM, Peter Cock <p.j.a.cock@googlemail.com> wrote:
On Thu, Apr 19, 2012 at 6:35 PM, JIE CHEN <jiechenable1987@gmail.com> wrote:
> Hi Peter,
>
> Thank you for your patience. I checked the error message in the history.
> They all give exactly the same error-- the one i gave in the first thread.

Are you saying this is the entire contents of the MIRA log entry in the history?

Return error code 1 from command:
mira --job=denovo,genome,accurate SANGER_SETTINGS
-LR:lsd=1:mxti=0:ft=fastq
-FN:fqi=/media/partition2_/galaxydb_data/000/dataset_290.dat
SOLEXA_SETTINGS -LR:lsd=1:ft=fastq
-FN:fqi=/media/partition2_/galaxydb_data/000/dataset_290.dat
COMMON_SETTINGS -OUT:orf=1:orc=1:ora=1:orw=1:orm=0:org=0:ors=0
-OUT:rrot=1:rtd=1

I'm pretty sure you are just telling me the error message. I would have expected
more than that, e.g. a line "MIRA took XXX minutes" before that error message.

To try to be even clearer:

1. Start your web browser and goto your Galaxy
2. Upload/import the files
3. Select the MIRA tool from left hand pane
4. Select input files and set parameters
5. Click "Execute"
6. Notice that six new history entries appear: MIRA contigs (FASTA),
MIRA contigs (QUAL)",MIRA contigs (CAF), MIRA contigs (ACE)", MIRA
coverage (Wiggle), MIRA log
7. Wait for MIRA to fail and the six new history entries to go red.
8. Click on the "eye" icon for the red history item "MIRA log"
9. Copy and paste the MIRA log contents to an email.

Also, and perhaps equally useful, can you access this server at the
command line and try the exact same failing command (from a temp
directory - it may create lots of files and folders)?

Peter