Hello, I installed Galaxy on Fedora 13. Everything works fine at http://127.0.0.1:8080 Then I followed instruction from https://bitbucket.org/galaxy/galaxy-central/wiki/Config/ApacheProxy. I add the following lines to httpd.conf: RewriteEngine on RewriteRule ^/galaxy$ /galaxy/ [R] RewriteRule ^/galaxy/static/style/(.*) /home/nate/galaxy_dist/static/june_2007_style/blue/$1 [L] RewriteRule ^/galaxy/static/scripts/(.*) /home/nate/galaxy_dist/static/scripts/packed/$1 [L] RewriteRule ^/galaxy/static/(.*) /home/nate/galaxy_dist/static/$1 [L] RewriteRule ^/galaxy/favicon.ico /home/nate/galaxy_dist/static/favicon.ico [L] RewriteRule ^/galaxy/robots.txt /home/nate/galaxy_dist/static/robots.txt [L] RewriteRule ^/galaxy(.*) http://localhost:8080$1 [P] and changed universe_wsgi.ini with [filter:proxy-prefix] use = egg:PasteDeploy#prefix prefix = /galaxy [app:main] filter-with = proxy-prefix cookie_path = /galaxy I restarted Apache and Galaxy. At http://127.0.0.1:8080 everything works fine but at http://xx.xx.xx.xx/galaxy I got a scrambled page. Apache error log said "[Mon Apr 11 17:43:03 2011] [error] [client xx.xx.xx.xx] (13)Permission denied: access to /galaxy/static/style/base.css denied, referer: http://xx.xx.xx.xx/galaxy/root/tool_menu [Mon Apr 11 17:43:03 2011] [error] [client xx.xx.xx.xx] (13)Permission denied: access to /galaxy/static/style/tool_menu.css denied, referer: http://xx.xx.xx.xx/galaxy/root/tool_menu [Mon Apr 11 17:43:03 2011] [error] [client xx.xx.xx.xx] (13)Permission denied: access to /galaxy/static/scripts/jquery.js denied, referer: http://xx.xx.xx.xx/galaxy/root/tool_menu [Mon Apr 11 17:43:03 2011] [error] [client xx.xx.xx.xx] (13)Permission denied: access to /galaxy/static/scripts/galaxy.base.js denied, referer: http://xx.xx.xx.xx/galaxy/root/tool_menu " Can anyone point out what was configured wrong? Many thanks in advance. Best regards, Yu Wang Yu Wang Office: Department of Plant Science Center for Life and Food Sciences Weihenstephan Technical University Munich Lehrstuhl für Pflanzenzüchtung Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt Technische Universität München Room 026, Emil-Ramann-Str. 4 (old: Am Hochanger 4) Tel: ++49 8161 71-4139 Lab: Lehrstuhl für Genomorientierte Bioinformatik Maximus-von-Imhof-Forum 3. Room OG-L 09 Tel: ++49 8161 71-2132 yu.wang@wzw.tum.de D-85354 Freising Germany