Hi, I have tried to follow instructions provided with this installation a) Run ./setup_deseq-hts.sh and setup paths and configuration options for DESeq-hts. I successfully ran the setup scrip but is there other path and conf that need to be done apart from those done by this script ? b) Inside the mex folder execute the make file to create platform dependent .mex files cd mex/Makefile make [interpreter] make octave for octave make matlab for matlab make all for octave and matlab When I run make octave I get the following error message : /usr/bin/mkoctfile -g --mex get_reads.cpp get_reads_direct.cpp mex_input.cpp read.cpp -I/mnt/galaxyTools/tools/samtools/0.1.16 -L/mnt/galaxyTools/tools/samtools/0.1.16 -lbam -lz -lcurses get_reads_direct.cpp:14:17: fatal error: sam.h: No such file or directory compilation terminated. make: *** [get_reads.mex] Error 1 It feels like this is link to samtools, when browsing on the web I found information about SAMTOOLS_ROOT env variable but changing this one doesn't solve my problem. As anyone else encounter this kind of things ? Regards, Philippe